TITLE

Development of a competitive liposome-based lateral flow assay for the rapid detection of the allergenic peanut protein Ara h1

AUTHOR(S)
Hsiao-Wei Wen; Borejsza-Wysocki, Wlodzimierz; DeCory, Thomas R.; Durst, Richard A.
PUB. DATE
July 2005
SOURCE
Analytical & Bioanalytical Chemistry;Jul2005, Vol. 382 Issue 5, p1217
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A competitive lateral flow assay for detecting the major peanut allergen, Ara h1, has been developed. The detector reagents are Ara h1-tagged liposomes, and the capture reagents are anti-Ara h1 polyclonal antibodies. Two types of rabbit polyclonal antibodies were raised either against the entire Ara h1 molecules (anti-Ara h1 Ab) or against an immunodominant epitope on Ara h1 (anti-peptide Ab). All of them reacted specifically with Ara h1 in Western Blot against crude peanut proteins. Moreover, the anti-Ara h1 Ab was chosen for this assay development because of its highest immunoactivity to Ara h1-tagged liposomes in the lateral flow assay. The calculated limit of detection (LOD) of this assay is 0.45 μg mL−1 of Ara h1 with a dynamic range between 0.1 and 10 μg mL−1 of Ara h1 in buffer. Additionally, the visually determined detection range is from 1 to 10 μg mL−1 of Ara h1 in buffer. Results using this assay can be obtained within 30 min without the need of sophisticated equipment or techniques; therefore, this lateral flow assay has the potential to be a cost-effective, fast, simple, and sensitive method for on-site screening of peanut allergens.
ACCESSION #
17551471

 

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