Mucosal colonisation with Lactobacillus casei mitigates barrier injury induced by exposure to trinitronbenzene sulphonic acid

Ijopis, M.; Antolín, M.; Guarner, F.; Salas, A.; Malagelado, J.-R.
July 2005
Gut;Jul2005, Vol. 54 Issue 7, p955
Academic Journal
Background: Trinitrobenzene sulphonic acid (TNBS) induces chronic transmural inflammatory lesions in the rat colon. Injury is facilitated by barrier disruption and invasion of commensal bacteria. However, certain bacteria have shown anti-inflammatory properties in in vitro models. Aim: To investigate in vivo the anti-inflammatory effect of Lactobacillus casei DN-114 001. Methods: Rats with a colonic segment excluded from faecal transit were surgically prepared. After washing the lumen with antibiotics, the excluded segment was recolonised (control group: standard flora of rat origin; test group: standard flora and L casei). Microbial colonisation was confirmed by culture of segment washing, and colitis was then induced by instillation of TNBS. One day after, intestinal lesions were blindly graded by macro- and microscopic scores, and myeloperoxidase activity measured in tissue homogenates. Translocation of bacteria to mesenteric lymph nodes, spleen and liver was investigated. Results: Test rats showed a smaller area of mucosal injury than control rats (p<0.05). Maximum depth lesion scores were similar in both groups but myeloperoxidase activity was lower in test than in control rats (p<0.05). Remarkably, bacterial translocation was quantitatively lower (p<0.01) and less frequent (p<0.05) in test than in control rats. Conclusion: In rats colonised with L casei, mucosal injury, inflammatory response, and barrier disruption after TNBS challenge were attenuated. Bacterial communities colonising the mucosa can modify inflammatory responses to luminal challenges.


Related Articles

  • Lactobacillus reuteri Maintains a Functional Mucosal Barrier during DSS Treatment Despite Mucus Layer Dysfunction. Dicksved, Johan; Schreiber, Olof; Willing, Ben; Petersson, Joel; Rang, Sara; Phillipson, Mia; Holm, Lena; Roos, Stefan; Chakravortty, Dipshikha // PLoS ONE;Sep2012, Vol. 7 Issue 9, Special section p1 

    Treatment with the probiotic bacterium Lactobacillus reuteri has been shown to prevent dextran sodium sulfate (DSS)- induced colitis in rats. This is partly due to reduced P-selectin-dependent leukocyte- and platelet-endothelial cell interactions, however, the mechanism behind this protective...

  • Regional difference in the healing process of colitis induced by 2,4,6-trinitrobenzene sulfonic acid in rats. Uchida, M.; Mogami, O.; Shimizu, K.; Matsueda, K. // Inflammopharmacology;Aug2007, Vol. 15 Issue 4, p158 

    We recently reported an improved method to induce colitis by 2,4,6-trinitrobenzene sulfonic acid. This method enabled us to induce colitis at appropriate regions. This study aimed to investigate the regional differences on the healing process of colitis in rats. Colitis was induced at the...

  • In vitro and in vivo characterization and strain safety of Lactobacillus reuteri NCIMB 30253 for probiotic applications. Sulemankhil, Imran; Parent, Mathieu; Jones, Mitchell Lawrence; Feng, Zhenqian; Labbé, Alain; Prakash, Satya // Canadian Journal of Microbiology;Jun2012, Vol. 58 Issue 6, p776 

    Lactobacillus reuteri NCIMB 30253 was shown to have potential as a probiotic by reducing the proinflammatory chemokine interleukin-8. Moreover, this strain was evaluated, by in vitro and in vivo techniques, for its safety for human consumption. The identity of the strain was investigated by...

  • BIOPROTECTIVE POTENTIAL OF BACTERIOCINS FROM SOME Lactobacillus species ISOLATED FROM FOODS. Oladipo, Iyabo Christianah; Anwooko, Deborah Oluyinka; Daramola, Dupe Ibukun; Babalola, Funmilayo Bose; Abidoye, Ganiyat T.; Olawale, Olaitan Dorcas; Iyanda, Kehinde Mary; Ajiboye, Ouwakemi Ruth; Osunpidan, Daniel Anuoluwapo; Ogunremi, Aminat Theresa; Olanbiwoninu, Afolake A. // Journal of Microbiology, Biotechnology & Food Sciences;Dec2016/Jan2017, Vol. 6 Issue 3, p900 

    Lactobacillus species isolated from ogi, kunnu, yoghurt and palm-wine were found to produce bacteriocins. The bacteriocins had broad spectra of antimicrobial activities against both Gram-positive and negative bacteria. The effects of the bacteriocins on Escherichia coli infections in rats were...

  • Hollis-Eden reports positive data for ulcerative colitis drug.  // PharmaWatch: Biotechnology;Nov2007, Vol. 6 Issue 11, p7 

    The article focuses on the benefit provided by HE3286 drug from Hollis-Eden Pharmaceuticals Inc. in wistar rat model of dinitrobenzene sulfonic acid induced colitis. It was found that HE3286-treated animals has reduced disease compared to placebo-controlled animals. It is considered that the...

  • Probiotics prevent bacterial translocation and improve intestinal barrier function in rats following chronic psychological stress. Zareie, M.; Johnson-Henry, K.; Jury, J.; Yang, P.-C.; Ngan, B.-Y.; McKay, D. M.; Soderholm, J. D.; Perdue, M. H.; Sherman, P. M. // Gut;Nov2006, Vol. 55 Issue 11, p1553 

    Background and aim: Chronic psychological stress, including water avoidance stress (WAS), induces intestinal mucosal barrier dysfunction and impairs mucosal defences against luminal bacteria. The aim of this study was to determine the ability of a defined probiotic regimen to prevent WAS induced...

  • Protective effect of aqueous extract of Oroxylum indicum Linn. (root bark) against DNBS-induced colitis in rats. Joshi, Shrikant V.; Vyas, Bhavin A.; Shah, Payal D.; Shah, Dinesh R.; Shah, Shailesh A.; Gandhi, Tejal R. // Indian Journal of Pharmacology;Dec2011, Vol. 43 Issue 6, p656 

    Objective: Aqueous root extract of Oroxylum indicum was evaluated in rats against dinitrobenzene sulfonic acid (DNBS) induced colitis. Materials and Methods: Rats were pre-treated orally for seven days and continued for four days after the induction of colitis with OIaq (100, 200, and 400 mg/kg)...

  • The effect of betulinic acid on TNBS-induced experimental colitis. Şener, Tarık Emre; Kardaş, Rıza Can; Şehirli, Ahmet Özer; Ekşioğlu-Demiral, Emel; Yüksel, Meral; Çetinel, Şule; Yeğen, Berrak C.; Şener, Göksel // Marmara Pharmaceutical Journal;Jan2013, Vol. 17 Issue 1, p52 

    In this study we have investigated the possible protective effect of betulinic acid (BA) on colonic inflammation in rats. Colitis was induced in Sprague-Dawley rats of both sexes by intracolonic administration of 1 ml trinitrobenzene sulphonic acid (TNBS). Colitisinduced rats received...

  • Protective effects of Aegle marmelos fruit pulp on 2,4,6-trinitrobenzene sulfonic acid-induced experimental colitis. Ghatule, Rohit R.; Gautam, Manish K.; Goel, Shalini; Singh, Amit; Joshi, Vinod K.; Goel, Raj K. // Pharmacognosy Magazine;Jan-Mar2014 Supplement, Vol. 10 Issue 37S, pS147 

    Background: Aegle marmelos (AM) fruit has been advocated in indigenous system of medicine for the treatment of various gastrointestinal disorders, fever, asthma, inflammations, febrile delirium, acute bronchitis, snakebite, epilepsy, leprosy, myalgia, smallpox, leucoderma, mental illnesses,...


Read the Article


Sign out of this library

Other Topics