TITLE

Fluorescence quenching in tip-enhanced nonlinear optical microscopy

AUTHOR(S)
Krug, John T.; Sánchez, Erik J.; Xie, X. Sunney
PUB. DATE
June 2005
SOURCE
Applied Physics Letters;6/6/2005, Vol. 86 Issue 23, p233102
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We describe the theoretical treatment of fluorescence quenching in tip-enhanced nonlinear optical microscopy (TENOM). Finite difference time domain simulations demonstrate that while sharp pyramidal probes yield fluorescence signal enhancement that decays monotonically as a function of probe-fluorophore distance, more commonly used conical probes cause more complex image contrast. Fluorescence quenching can thus explain the halo-type images that are sometimes observed in TENOM. Formation of a dielectric spacer layer on the TENOM probe should alleviate the complications associated with quenching.
ACCESSION #
17328477

 

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