TITLE

Rapid analysis of pyrethroids in whole urine by high-performance liquid chromatography using a monolithic column and off-line preconcentration in a restricted access material cartridge

AUTHOR(S)
León-González, M. E.; Plaza-Arroyo, M.; Pérez-Arribas, L. V.; Polo-Díez, L. M.
PUB. DATE
May 2005
SOURCE
Analytical & Bioanalytical Chemistry;May2005, Vol. 382 Issue 2, p527
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A rapid high-performance liquid chromatography (HPLC) method using a monolithic column with UV detection at 238 nm was developed for the determination of fenpropathrin, betacyfluthrin, deltamethrin, and permethrin ( cis and trans isomers) in whole urine. The method is based on the use of a monolithic chromatographic column and a restricted access material (RAM) cartridge for sample preparation. The mobile phase was water/acetonitrile (42:58 v/v), the flow rate was 3 mL min−1, and chromatographic separation was carried out in 10 min. The separation of cis and trans isomers of permethrin was also possible under the above-mentioned conditions. Detection limits in reconstituted whole urine samples were between 0.9 μg L−1 for betacyfluthrin and 4.4 μg L−1 for fenpropathrin and trans-permethrin. Recoveries for urine samples spiked with different amounts of pyrethroids (between 19 μg L−1 and 75 μg L−1) were in the 70±6 to 90±7% range.
ACCESSION #
17252661

 

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