May 2005
Thorax;May2005, Vol. 60 Issue 5, p409
Academic Journal
The article states that molecular techniques improve organism identification from pleural fluid in children with empyema, even after commencement of antibiotics, but should be considered complementary to culture. The study compares a broad range molecular technique with bacterial culture for the detection of organisms from pleural fluid in 32 children with empyema. The molecular assay is an established and validated broad range 16S rDNA PCR technique. This is based on bacterial ribosomal rDNA with sequencing of the PCR product to reveal the source organism.


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