TITLE

Determination of levodopa methyl ester and its metabolites in rat serum by CZE with amperometric detection

AUTHOR(S)
Wang, J.; Zhou, Y.; Wang, A. F.; Zhang, S.H.; Ning, L.; He, P. G.; Fang, Y. Z.
PUB. DATE
May 2005
SOURCE
Analytical & Bioanalytical Chemistry;May2005, Vol. 382 Issue 1, p198
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A reliable and reproducible method, capillary zone electrophoresis with amperometric detection (CZE-AD), has been developed for separation and quantification of levodopa methyl ester (LDME) and its biotransformation products levodopa (L-DOPA) and dopamine (DA) in rat serum. A carbon-disk electrode was used as working electrode. The optimum conditions for CZE detection were 50 mmol L-1 phosphate solution at pH 7.0 as running buffer, 17 kV as separation voltage, 1.0 V (vs Ag/AgCl, 3.0 mol L-1) as detection potential, and sample injection for 8 s at 17 kV. The linear ranges were from 2.4×10-2 to 2.2 µg mL-1 for LDME, 2.9×10-1 to 49.5 µg mL-1 forL-DOPA, and 1.4×10-2 to 1.5 µg mL-1 for DA with correlation coefficients of 0.9997, 0.9994, and 0.9999, respectively. The detection limits for LDME,L-DOPA, and DA were 14.6, 98.0, and 9.7 ng mL-1, respectively. Recoveries were 80.3% for LDME, 93.5% forL-DOPA, and 86.5% for DA. This method was applied to serum samples after intravenous injection of LDME andL-DOPA to rats.
ACCESSION #
17051993

 

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