TITLE

Immunocytochemical identification of CD5 positive peripheral blood lymphocytes in four marsupial species

AUTHOR(S)
Young, L.J.; Deane, E.M.
PUB. DATE
May 2003
SOURCE
Comparative Clinical Pathology;May2003, Vol. 12 Issue 1, p26
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Immunocytochemical analysis of peripheral blood mononuclear cells was undertaken using a streptavidin biotin–horseradish peroxidase method to detect CD5 positive lymphocytes from the blood of several marsupial species. A monoclonal antibody raised to a conserved peptide sequence of the human CD5 antigen positively labelled lymphocytes in freshly isolated peripheral blood mononuclear cells of the tammar wallaby (Macropus eugenii), the long-footed potoroo (Potorous longipes), the long-nosed potoroo (Potorous tridactylus) and the rufous hare-wallaby (Lagorchestes hirsutus). A polyclonal anti-CD3 antibody also positively labelled circulating lymphocytes from the tammar wallaby. Whereas previous studies using flow cytometry reported labelling of T cells in koala lymphocyte preparations using a polyclonal anti-CD3 antibody, there have been no other reports of marsupial blood immunophenotyping. The current study extends the known applications of monoclonal anti-CD5 and polyclonal anti-CD3 antibodies to blood lymphocytes of small wallaby species using an immunocytochemical slide technique that is simple, can be processed within a day and requires no dedicated large equipment.
ACCESSION #
17043400

 

Related Articles

  • Monoclonal Antibody--Defined Lymphocyte Subsets in Normal Nigerians. Adedeji, M. O. // Angiology;Nov1989, Vol. 40 Issue 11, p977 

    Lymphocyte subsets using monoclonal antibodies have hitherto not been reported in normal Nigerian subjects. In all 8 subjects, the average number of lymphocytes showed a clear rhythmicity with higher values in the morning (8 AM) than in the afternoon (2 PM). The time of day variation in...

  • T cell activation alters intestinal structure and function. Field, Michael // Journal of Clinical Investigation;Oct2006, Vol. 116 Issue 10, p2580 

    Treatment with anti-CD3 antibody (anti-CD3) causes transient diarrhea. In this issue of the JCI, Clayburgh et al. show that, in jejunum of mice injected with anti-CD3 or with TNF, fluid accumulation and changes in epithelial phenotype develop, the latter including an increase in the passive...

  • To be or not to be B7. Xingxing Zang; Allison, James P. // Journal of Clinical Investigation;Oct2006, Vol. 116 Issue 10, p2590 

    The activation of lymphocytes and development of adaptive immune responses is initiated by the engagement of TCRs by antigenic peptide—MHC complexes and shaped at the clonal level by both positive and negative costimulatory signals. The B7 family members are involved at several stages in...

  • A critical review of the role of Fc gamma receptor polymorphisms in the response to monoclonal antibodies in cancer. Mellor, James D.; Brown, Michael P.; Irving, Helen R.; Zalcberg, John R.; Dobrovic, Alexander // Journal of Hematology & Oncology;2013, Vol. 6 Issue 1, p1 

    Antibody-dependent cellular cytotoxicity (ADCC) is a major mechanism of action of therapeutic monoclonal antibodies (mAbs) such as cetuximab, rituximab and trastuzumab. Fc gamma receptors (FcgR) on human white blood cells are an integral part of the ADCC pathway. Differential response to...

  • A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity. Tatsuhiko Ozawa; Xiuhong Piao; Kobayashi, Eiji; Yue Zhou; Sakurai, Hiroaki; Andoh, Tsugunobu; Jin, Aishun; Kishi, Hiroyuki; Muraguchi, Atsushi // PLoS ONE;Dec2012, Vol. 7 Issue 12, p1 

    Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system using immunospot array assay on a chip...

  • The Relationship between T Lymphocyte Subsets and Ia-like Antigen Positive Nonlymphoid Cells in Early Stages of Cutaneous T Cell Lymphoma. Thomas, J. Alero; Janossy, George; Graham-Brown, Robin A. C.; Kung, Patrick C.; Goldstein, Gideon // Journal of Investigative Dermatology;Feb82, Vol. 78 Issue 2, p169 

    Immunofluorescence studies were carried out in cutaneous T cell lymphoma (mycosis fungoides) in order to analyse the microanatomical relationship of the different T lymphocyte subsets (inducer and suppressor/cytotoxic cell populations) to large nonlymphoid Langerhans-type and so-called...

  • Immune Disorders: Oral antibody against autoimmunity. Flemming, Alexandra // Nature Reviews Drug Discovery;Aug2006, Vol. 5 Issue 8, p631 

    The article examines the effect of the oral delivery of monoclonal antibodies on treating autoimmune diseases. It was found that the low dose of antibody can suppress the onset of the disease. Oral monoclonal antibodies does not modulate the T-cell receptor-CD3 complex on T cells. It exerts a...

  • The p24 leucocyte membrane antigen: Modulation associated with lymphocyte activation and differentiation. Zola, H.; Furness, V.; Barclay, S.; Zowtyj, H.; Smith, M.; Melo, J. V.; Neoh, S. H.; Bradley, J. // Immunology & Cell Biology;Feb1989, Vol. 67 Issue 1, p63 

    Monoclonal antibodies of the CD9 cluster recognize a 24 kD protein (p24) found on platelets and endothelium. and expressed by lymphocytes at restricted stages of maturation and activation. In this study, we explore the possibility that p24 is involved in the response of lymphocytes to signals...

  • Artifacts Arising from Using Leukocytic Fc Receptor Blocking Buffer. Zubova, S.; Kabanov, D.; Serov, D.; Grachev, S.; Prokhorenko, I. // Bulletin of Experimental Biology & Medicine;Dec2016, Vol. 162 Issue 2, p244 

    We studied the effects of Human TruStain FcX buffer (Fcγ receptor blocking solution) in experiments on evaluation of TLR4 level with labeled monoclonal antibodies, intracellular immunofluorescent staining of NF-κB p50, and TNF-α synthesis on human isolated monocytes and whole blood...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics