Expression of functional protease-activated receptor 1 in human prostate cancer cell lines

Jian Liu; Bastian, Manuela; Kohlschein, Peter; Schuff-Werner, Peter; Steiner, Michael
July 2003
Urological Research;Jul2003, Vol. 31 Issue 3, p163
Academic Journal
Functional protease-activated receptors (PAR) are expressed by a variety of malignant cells. In the present study, RT-PCR assays demonstrated the expression of the thrombin receptor PAR-1 mRNA in human prostate cancer cell lines DU 145, LnCAP, and SV40-immortalized human prostate epithelial cell line PNT1A. In contrast, the additional thrombin receptors PAR-3 and PAR-4 were not detected. PAR-1 protein localized to the cellular surface was detected by flow cytometry in all three cell lines. To demonstrate the functional importance of the PAR-1, the effects of different concentrations of thrombin on cell proliferation kinetics were assessed. The treatment of growth-arrested cells with varying concentrations of thrombin demonstrated dose- and time-dependent effects. At low concentration (<0.5 U/ml), thrombin induced proliferation of all prostate-derived cell lines. Thrombin at higher concentration (1.0 U/ml) initially stimulated PNT1A and LnCAP cells to proliferate (time of thrombin application 24 h and 48 h) followed by inhibited growth when assessed after 72 h of incubation. In contrast, 1.0 U/ml thrombin caused earlier inhibition of DU 145 proliferation starting at 48 h of incubation. Our results suggest that PAR-1 mediates the proliferation-modulating effects of thrombin on prostate cancer cells.


Related Articles

  • Proliferative Tumor Doubling Times of Prostatic Carcinoma. Werahera, Priya N.; Glode, L. Michael; La Rosa, Francisco G.; Lucia, M. Scott; Crawford, E. David; Easterday, Kenneth; Sullivan, Holly T.; Sidhu, Rameshwar S.; Genova, Elizabeth; Hedlund, Tammy // Prostate Cancer (20903111);2011, p1 

    Prostate cancer (PCa) has a variable biology ranging from latent cancer to extremely aggressive tumors. Proliferative activities of cancers may indicate their biological potential. A flow cytometric assay to calculate maximum proliferative doubling times (Tmax) of PCa in radical prostatectomy...

  • Anti-tumor activity of the TRPM8 inhibitor BCTC in prostate cancer DU145 cells. TAO LIU; ZHIHAI FANG; GANG WANG; MINGJUN SHI; XIAO WANG; KUN JIANG; ZHONGHUA YANG; RUI CAO; HUANGHENG TAO; XINGHUAN WANG; JIAJIE ZHOU // Oncology Letters;Jan2016, Vol. 11 Issue 1, p182 

    The present study investigated the anti-tumor activity of N-(4-tertiarybutylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carbox-amide (BCTC), a potent and specific inhibitor of transient receptor potential cation channel subfamily M member 8 (TRPM8) in prostate cancer (PCa) DU145...

  • Dual activities of galectin-3 in human prostate cancer: tumor suppression of nuclear galectin-3 vs tumor promotion of cytoplasmic galectin-3. Califice, Stéphane; Castronovo, Vincent; Bracke, Marc; van den Brûle, Frédéric // Oncogene;9/30/2004, Vol. 23 Issue 45, p7527 

    Galectin-3, a multifunctional lectin, is involved during cancer progression. Previous observations showed that both cytosolic expression and nuclear exclusion of galectin-3 in human prostate cancer cells were associated to progression of the disease. In this study, we examined the biological...

  • MDM2 antagonists boost antitumor effect of androgen withdrawal: implications for therapy of prostate cancer. Tovar, Christian; Higgins, Brian; Kolinsky, Kenneth; Mingxuan Xia; Packman, Kathryn; Heimbrook, David C.; Vassilev, Lyubomir T. // Molecular Cancer;2011, Vol. 10 Issue 1, p49 

    Background: Hormone therapy is the standard of care for newly diagnosed or recurrent prostate cancers. It uses anti-androgen agents, castration, or both to eliminate cancer promoting effect of testicular androgen. The p53 tumor suppressor controls a major pathway that can block cell...

  • Effects of DNAzymes targeting Aurora kinase A on the growth of human prostate cancer. Qu, Y; Zhang, L; Mao, M; Zhao, F; Huang, X; Yang, C; Xiong, Y; Mu, D // Cancer Gene Therapy;Aug2008, Vol. 15 Issue 8, p517 

    Aurora kinase A has been demonstrated to be involved in the malignant progression of many types of cancer including prostate cancer, we therefore hypothesized that Aurora kinase A might work as a valuable target for prostate cancer treatment. To test this hypothesis, we used DNAzyme technology...

  • BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability. Kuo, P-C; Huang, C-W; Lee, C-I; Chang, H-W; Hsieh, S-W; Chung, Y-P; Lee, M-S; Huang, C-S; Tsao, L-P; Tsao, Y-P; Chen, S-L // British Journal of Cancer;1/20/2015, Vol. 112 Issue 2, p391 

    Background:We showed previously that breast carcinoma amplified sequence 2 (BCAS2) functions as a negative regulator of p53. We also found that BCAS2 is a potential AR-associated protein. AR is essential for the growth and survival of prostate carcinoma. Therefore we characterised the...

  • Immunohistochemical characterisation of the monoclonal antibody BLCA-38 for the detection of prostate cancer. Russell, P.J.; Ow, K.T.; Tam, P.N.; Juarez, J.; Kingsley, E.A.; Qu, C.F.; Li, Y.; Cozzi, P.J.; Martiniello-Wilks, R. // Cancer Immunology, Immunotherapy;Nov2004, Vol. 53 Issue 11, p995 

    Background: Monoclonal antibodies (MAbs) can be used to detect, image and treat cancers. This study aimed to characterise the binding of BLCA-38 MAbs to human prostate cancer cell lines, human prostate cancer biopsy samples and normal tissues to enable future targeted studies. Methods: BLCA-38...

  • Inhibition of hedgehog signaling reduces the side population in human malignant mesothelioma cell lines. Kim, H-A; Kim, M-C; Kim, N-Y; Kim, Y // Cancer Gene Therapy;Aug2015, Vol. 22 Issue 8, p387 

    Deregulation of crucial embryonic pathways, including hedgehog signaling, has been frequently implicated in a variety of human cancers and is emerging as an important target for anticancer therapy. This study evaluated the potential anticancer effects of cyclopamine, a chemical inhibitor of...

  • 顺铂作用于人肝癌细胞系HepG2后肿瘤干细胞标志物增加. 赵小鸽; 田美丽; 杨阳; 童冬冬; 陈伟 // Journal of Xi'an Jiaotong University (Medical Sciences);Jan2015, Vol. 36 Issue 1, p89 

    Objective To investigate the changes of HepG2 cell proliferation and cycle as well as changes of CD133, ABCG2 and ICAM-1 positive cells proportion in the presence of cisplatin on human hepatocellular carcinoma HepG2 cell line. Methods MTT colorimetric assay was used to observe the effects of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics