Octreotide induced prolongation of colonic transit increases faecal anaerobic bacteria, bi!e acid metabolising enzymes, and serum deoxycholic acid in patients with acromegaly

Thomas, L. A.; Veysey, M J; Murphy, G M; Russell-Jones, D; French, G L; Wass, J A H; Dowling, R H
May 2005
Gut;May2005, Vol. 54 Issue 5, p630
Academic Journal
Background: Acromegalic patients have slow colonic transit, increased rates of deoxycholic acid formation, and an increased prevalence of cholesterol gall stones, especially during long term octreotide treatment. However, the effects of this prolonged large bowel transit time on the numbers of faecal anaerobes and the activities of the enzyme systems which biotransform conjugated cholic acid into unconjugated deoxycholic acid (cholylglycine hydrolase and 7α-dehydroxylase) are unknown. Methods: Therefore, in 10 non-acromegalic controls, 11 acromegalic patients not treated with octreotide, and 11 acromegalics on long term (8-48 months) octreotide (100-200 μg three times daily subcutaneously), we measured large bowel transit time and, in freshly voided Faeces, the activities of the two bile acid metabolising enzymes, and related the results to the proportion of deoxycholic acid in fasting serum. Moreover, in patients with acromegaly, we measured quantitative bacteriology in faeces. Results: Mean large bowel transit time in acromegalics not treated with octreotide (35 (SEM 6.5) hours) was 66% longer than that in non-cicromegalic controls (21 (3.1) hours; NS) and became further prolonged during octreotide treatment (48 (6.6) hours; p<0.001). These octreotide induced changes in transit were associated, in acromegalic patients, with more total (15.0 (2.5) v 6.3 (1.3)×0.059 colony forming units (cfu)/g; p<10.05) and Gram positive (6.3 (2.3) v 3.2 1.0) × 109 cfu/g;p<0.05) faecal anaerobes. Mean faecal cholyiglycine hydrolase activity in the long term octreotide group (22.0 (6.0) × 10-2 U/mg protein) was 138% greater than that in non-acromegalic controls (12.0 (6.0) ×10-2; pc<0.01). Similarly, mean 7α-dehydroxylase activity in octreotide treated acromegalics (11.1(1.18) × 10-4 U/mg protein) was 78% greater than that in patients not receiving long term octreotide (6.3 (0.5) × 10-4; p<0.001). The mean proportion of deoxycholic acid in fasting serum also increased from 18.0 (2.88)% in the untreated group to 29.6 (2.3)% during long term octreotide (p<0.05). There were significant linear relationships between large bowel transit time and: (i) faecal 7α-dehydroxylase activity; and (ii) the proportion of deoxycholic acid in fasting serum and between 7α-dehydroxylase activity and the proportion of deaxycholic acid in serum.


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