TITLE

Rapid and sensitive method for the determination of acetaldehyde in fuel ethanol by high-performance liquid chromatography with UV-Vis detection

AUTHOR(S)
Saczk, Adelir Aparecida; Okumura, Leonardo Luiz; De Oliveira, Marcelo Firmino; Zanoni, Maria Valnice Boldrin; Stradiotto, Nelson Ramos
PUB. DATE
April 2005
SOURCE
Analytical & Bioanalytical Chemistry;Apr2005, Vol. 381 Issue 8, p1619
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A high-performance liquid chromatography (HPLC) method for the determination of acetaldehyde in fuel ethanol was developed. Acetaldehyde was derivatized with 0.900 mL 2,4-dinitrophenylhydrazine (DNPHi) reagent and 50 µL phosphoric acid 1 mol L-1 at a controlled room temperature of 15°C for 20 min. The separation of acetaldehyde-DNPH (ADNPH) was carried out on a Shimadzu Shim-pack C18 column, using methanol/LiCl(aq) 1.0 mM (80/20, v/v) as a mobile phase under isocratic elution and UV-Vis detection at 365 nm. The standard curve of ADNPH was linear in the range 3-300 mg L-1 per injection (20 µL) and the limit of detection (LOD) for acetaldehyde was 2.03 µg L-1, with a correlation coefficient greater than 0.999 and a precision (relative standard deviation, RSD) of 5.6% (n=5). Recovery studies were performed by fortifying fuel samples with acetaldehyde at various concentrations and the results were in the range 98.7-102%, with a coefficient of variation (CV) from 0.2% to 7.2%. Several fuel samples collected from various gas stations were analyzed and the method was successfully applied to the analysis of acetaldehyde in fuel ethanol samples.
ACCESSION #
16865353

 

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