TITLE

Determination of sterols in biological samples by SPME with on-fiber derivatization and GC/FID

AUTHOR(S)
Domeño, Celia; Ruiz, Bárbara; Nerín, Cristina
PUB. DATE
April 2005
SOURCE
Analytical & Bioanalytical Chemistry;Apr2005, Vol. 381 Issue 8, p1576
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A new procedure for the determination of sterols in serum samples is proposed. The system consists of coating a Solid Phase Microextraction (SPME) microfiber in headspace mode with the derivatizing agentN,O-bis(trimethylsilyl)trifluoracetamide (BSTFA) and then applying this coated fiber to the simultaneous extraction and derivatization of three precursors in the cholesterol biosynthesis pathway (desmosterol, lathosterol and lanosterol) and two phytosterols (sitosterol and sitostanol) in serum samples. Optimization of the analytical procedure via the application of an experimental design, a study of matrix effects, and an analysis of serum pool samples are all described and discussed.
ACCESSION #
16865337

 

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