TITLE

Purification and Characterization of an Extracellular Non-Aspartyl Acid Protease (pumAe) from Ustilago maydis

AUTHOR(S)
Mercado-Flores, Yuridia; Guerra-Sánchez, Guadalupe; Villa-Tanaca, Lourdes; Hernández-Rodríguez, César
PUB. DATE
October 2003
SOURCE
Current Microbiology;Oct2003, Vol. 47 Issue 5, p408
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The proteinase pumAe was purified to homogeneity from haploid U. maydis FB1 growing on acid mineral medium. The purification procedure consisted of ammonium sulfate fractionation and gel filtration chromatography, resulting in a 7.7% recovery and a 15.1-fold increase in specific activity. The molecular weight of the enzyme was estimated to be 72 kDa and 74 kDa by gel filtration chromatography and SDS-PAGE, respectively. Enzymatic activity was optimal at pH 4.0 and at 45°C toward hemoglobin, and the pI was determined to be 5.5. The effects of six protease inhibitors on pumAe were tested, and no inhibitory effect was observed. The pure enzyme degraded gelatin and albumin, but casein and collagen were not degraded. The Km value was 3.5 μM, and the Vmax value was 11430 μmol h-1 mg-1 for Suc-R-P-F-H-L-L-V-Y-MCA.
ACCESSION #
16859941

 

Related Articles

  • The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity. Brundiek, Henrike; Saß, Stefan; Evitt, Andrew; Kourist, Robert; Bornscheuer, Uwe // Applied Microbiology & Biotechnology;Apr2012, Vol. 94 Issue 1, p141 

    The Ustilago maydis lipase UM03410 belongs to the mostly unexplored Candida antarctica lipase (CAL-A) subfamily. The two lipases with [...] the highest identity are a lipase from Sporisorium reilianum and the prototypic CAL-A. In contrast to the other CAL-A-type lipases, this hypothetical U....

  • Inverse pH Regulation of Plant and Fungal Sucrose Transporters: A Mechanism to Regulate Competition for Sucrose at the Host/Pathogen Interface? Wippel, Kathrin; Wittek, Anke; Hedrich, Rainer; Sauer, Norbert // PLoS ONE;2010, Vol. 5 Issue 8, p1 

    Background: Plant sucrose transporter activities were shown to respond to changes in the extracellular pH and redox status, and oxidizing compounds like glutathione (GSSG) or H2O2 were reported to effect the subcellular targeting of these proteins. We hypothesized that changes in both parameters...

  • Farming fungus in Florida. Werner, Louis // Americas;Jul/Aug98, Vol. 50 Issue 4, p4 

    Focuses on the cultivation of corn fungus or ustilago maydis in the United States. Advantages and disadvantages of corn fungus; Details on efforts to popularize ustilago maydis in the United States; Cultivation of corn fungus by farmer Roy Burns from Groveland, Florida; How is corn fungus...

  • A MADS-box homologue in Ustilago maydis regulates the expression of pheromone-inducible... Kruger, Julia; Aichinger, Christian // Genetics;Dec97, Vol. 147 Issue 4, p1643 

    Discusses the sexual development of pathogenic fungus, namely Ustilago maydis. How is this development controlled; Identification of the pheromone response factor Prflp; Mediation of Prflp; Description of the isolation of genes.

  • SCOOPS. Moss, Meg // Ask;Oct2010, Vol. 9 Issue 8, p2 

    The article presents an update on issues related to science as of October 2010, including one on health benefits of a fungus called huitlacoche or corn smut, another on Chariot Skates designed by an Australian inventor and finally, on a key role that might be played by whales and their feces in...

  • Characterization of a new aryl-alcohol oxidase secreted by the phytopathogenic fungus Ustilago maydis. Couturier, Marie; Mathieu, Yann; Li, Ai; Navarro, David; Drula, Elodie; Haon, Mireille; Grisel, Sacha; Ludwig, Roland; Berrin, Jean-Guy // Applied Microbiology & Biotechnology;Jan2016, Vol. 100 Issue 2, p697 

    The discovery of novel fungal lignocellulolytic enzymes is essential to improve the breakdown of plant biomass for the production of second-generation biofuels or biobased materials in green biorefineries. We previously reported that Ustilago maydis grown on maize secreted a diverse set of...

  • A proposed mechanism for the inhibitory effect of the anticancer agent titanocene dichloride on tumour gelatinases and other proteolytic enzymes. Pavlaki, Maria; Debeli, Katerina; Triantaphyllidou, Irene-Eva; Klouras, Nikolaos; Giannopoulou, Eleftheria; Aletras, Alexios J. // Journal of Biological Inorganic Chemistry;Jul2009, Vol. 14 Issue 6, p947 

    Titanocene dichloride, the most studied metallocene, exhibits antiproliferative activity in a wide spectrum of murine and human tumours. In this article it is demonstrated that titanocene dichloride inhibits tumour gelatinases in a dose-dependent manner. Substrate saturation experiments and the...

  • Optimization of extracellular thermotolerant alkaline protease produced by marine Roseobacter sp. (MMD040). S. Shanmughapriya; J. Krishnaveni; Joseph Selvin; R. Gandhimathi; M. Arunkumar; T. Thangavelu; G. Kiran; K. Natarajaseenivasan // Bioprocess & Biosystems Engineering;Aug2008, Vol. 31 Issue 5, p427 

    Abstract  Marine endosymbiontic Roseobacter sp. (MMD040), which produced high yields of protease, was isolated from marine sponge Fasciospongia cavernosa, collected from the peninsular coast of India. Maximum production of enzyme was obtained in Luria-Bertani broth. Catabolite repression...

  • Mutations in the myp1 gene of Ustilago maydis attenuate mycelial growth and virulence. Giasson, Luc; Kronstad, James W. // Genetics;Oct95, Vol. 141 Issue 2, p491 

    Describes the construction and insertional mutagenesis of a haploid strain of a Ustilago maydis mutant gene with a mycelial phenotype (myp-1). Isolation and sequence analysis of myp1; Disruption or deletion mutations in the myp-1 gene; Role of myp-1 in mycelial growth and virulence.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics