Enhanced Tumor Necrosis Factor-α Expression in Small Sized Abdominal Aortic Aneurysms World J. Surg. Vol. 27, No. 4 April 2003

Hamano, Kimikazu; Li, Tao-Sheng; Takahashi, Mutsuo; Kobayashi, Toshiro; Shirasawa, Bungo; Ito, Hiroshi; Zempo, Nobuya
April 2003
World Journal of Surgery;Apr2003, Vol. 27 Issue 4, p476
Academic Journal
Circulating levels of tumor necrosis factor α (TNF-α) are elevated in the patients with abdominal aortic aneurysm (AAA). We investigated TNF-α expression and cellular infiltration in the walls of AAAs of different sizes. Twenty-seven surgical specimens of AAAs were categorized according to the maximum aneurysm diameter into a small size group (less than 50 mm in diameter, n = 8; S group), a medium-sized group (50 to 59 mm in diameter, n = 11; M group), and a large size group (larger than 59 mm in diameter, n = 8; L group). The level of TNF-α and interleukin-1β(IL-1β) in the aneurysm wall was measured by ELISA. Immunohistochemical staining was performed to observe the TNF-α expression and the infiltration of macrophages and lymphocytes in aneurysm walls. Enzyme-linked immunosorbent assay showed that the level of TNF-α in the S group (5.47 ± 3.48 pg/mg protein) was significantly higher (p < 0.05) than that in the M group (2.70 ± 1.33 pg/mg protein) or the L group (1.82 ± 1.21 pg/mg protein). No significant difference in IL-1β was observed between the S, M, and L groups. Immunohistochemical analysis also showed that TNF-α was expressed strongly in the S group but was negative or weakly positive in the M and L groups. Furthermore, the expression of TNF-α was seen mainly where the aneurysm wall showed atheromatous change and macrophage infiltration. These results indicated that the expression of TNF-α in the aneurysm wall was enhanced in small AAAs, and this enhancement might be related to the infiltration of macrophages.


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