TITLE

Origin, Evolution, and Metabolic Role of a Novel Glycolytic GAPDH Enzyme Recruited by Land Plant Plastids

AUTHOR(S)
Petersen, Jörn; Brinkmann, Henner; Cerff, Rüdiger
PUB. DATE
July 2003
SOURCE
Journal of Molecular Evolution;Jul2003, Vol. 57 Issue 1, p16
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
NAD-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a cytosolic marker enzyme of eukaryotes (GapC; EC 1.2.1.12). Land plants possess an additional NADP+-dependent enzyme (EC 1.2.1.13) within their chloroplasts which is composed of two subunits, GapA and GapB. Another plastid GAPDH enzyme (GapCp) was recently discovered in gymnosperms and ferns. This novel GapCp is closely related to cytosolic GapC and displays glycolytic NAD+ cosubstrate specificity. Here we show that this new gene GapCp is also present and actively expressed in angiosperms, mosses, and liverworts. Phylogenetic analyses of the available GapC and GapCp sequences suggest that the gene duplication giving rise to GapCp occurred in ancestral charophyte algae. The data are also consistent with a monophyletic origin of charophytes and land plants and further support the view that land plants arose from a Coleochaete-like green alga. Northern hybridizations were employed to study the expression of the genes GapCp, GapC, GapA, and GapB in green and nongreen tissues from pepper (Capsicum annuum). The results demonstrate that GapCp mRNAs are mainly expressed in red pepper fruit and roots, in which the transcript levels of photosynthetic GapA and GapB are downregulated. This suggests that in flowering plants GapCp plays a specific role in glycolytic energy production of nongreen plastids such as chromoplasts and leukoplasts and that angiosperms may be the only land plants where glycolysis is absent in green chloroplasts.
ACCESSION #
16766724

 

Related Articles

  • In This Issue.  // Clinical Pharmacology & Therapeutics;Feb2016, Vol. 99 Issue 2, p123 

    An introduction is presented which focuses on topics including deficiency in dihydropyrimidine dehydrogenase, drug-induced long QT syndrome, and biomarkers.

  • gink·go.  // American Heritage Student Science Dictionary;2009, p150 

    The entry provides information on ginkgo, a deciduous tree originally native to China. It has fan-shaped leaves and fleshy yellow seeds.

  • Stem-righting Mechanism in Gymnosperm Trees Deduced from Limitations in Compression Wood Development. Saori Yamashita; Masato Yoshida; Shozo Takayama; Takashi Okuyama // Annals of Botany;Mar2007, Vol. 99 Issue 3, p487 

    Background and Aims In response to inclination stimuli, gymnosperm trees undergo corrective growth during which compression wood develops on the lower side of the inclined stem. High compressive growth stress is generated in the compression wood region and is an important factor in righting the...

  • EL ENDEMISMO EN LA FLORA FANEROGAMICA MEXICANA: UNA APRECIACION ANALITICA PRELIMINARY. Rzedowski, Jerzy // Acta Botanica Mexicana;sep1991, Issue 15, p47 

    This contribution is essentially a complement to the data and concepts exposed in a previous paper, devoted to the topic of diversity and origins of the Mexican phanerogamic flora. A part of the mentioned paper deals with the subject of endemism which, according to evidence and estimations,...

  • Taxonomic and floristic note on two Southamerican species of Ephedra (Ephedraceae). Xifreda, Cecilia Carmen; Hunziker, Juan Hector // Darwiniana;1987, Vol. 28 Issue 1-4, p413 

    Ephedra andina C. Meyer is considered to be a synonym of E. chilensis C. Presl; the latter binomial having been considered earlier as a "nomen dubium" is rehabilitated. The presence of E. multiflora Stapf in Chile is breafly discussed and is supported by an exsiccata collected at the Antofagasta...

  • Enzymatic and histopathologic biomarkers as indicators of aquatic pollution in fishes. Osman, Alaa G. M.; Abd El Reheem, Abd-El –Baset M.; AbuelFadl, Khalid Y.; GadEl-Rab, Ali G. // Natural Science;Nov2010, Vol. 2 Issue 11, p1302 

    In the present study we investigated the alteration in the activity of two metabolic enzymes [Glucose-6-Phosphate Dehydrogenase (G6PDH) and Lactate Dehydrogenase (LDH)] and the histological changes on liver and gills of the African catfish Clarias gariepinus collected from 6 sites along the...

  • An In Vitro Test to Screen Skin Sensitizers Using a Stable THP-1–Derived IL-8 Reporter Cell Line, THP-G8. Takahashi, Toshiya; Kimura, Yutaka; Saito, Rumiko; Nakajima, Yoshihiro; Ohmiya, Yoshihiro; Yamasaki, Kenshi; Aiba, Setsuya // Toxicological Sciences;Dec2011, Vol. 124 Issue 2, p359 

    Several studies have suggested that interleukin (IL)-8 can serve as a biomarker for discrimination of skin sensitizers from nonsensitizers. We established a stable THP-1–derived IL-8 reporter cell line, THP-G8, which harbors SLO and SLR luciferase genes under the control of IL-8 and...

  • Valoración diagnóstica de enzimas hepáticas en perfiles bioquímicos sanguíneos de vacas lecheras. Noro, Mirela; Cid T., Pía; Wagemann F., Catalina; Arnés V., Veronica; Wittwer M., Fernando // Revista MVZ Córdoba;may-ago2013, Vol. 18 Issue 2, p3474 

    Objective. The aim of this study was to establish the association, sensibility (Se) and specificity (Sp) of the plasmatic activity of glutamate dehydrogenase (GDH, EC: 1.4,1.3), aspartate aminotransferase (AST, EC: 2.6.1.1) and g-glutamil transpeptidase (GGT, EC: 2.3.2.2) as liver damage markers...

  • Carboplatin-mediated changes in the activity levels of steroidogenic marker enzymes, serum hormones and testicular architecture in male rats. Reddy, Y. V. Kishore; Reddy, P. Sreenivasula; Shivalingam, M. R.; Devi, B. Suvarchala; Pravallika, G.; Anusha, D. Naga // Drug Invention Today;Jan2010, Vol. 2 Issue 1, p42 

    Carboplatin (10 mg/ kg body weight) caused significant decrease in the activity levels of steroidogenic marker enzymes such as 3β-hydroxy steroid dehydrogenase and 17β-hydroxy steroid dehydrogenase in experimental rats when compared to that of the control rats. In addition, activity levels...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics