TITLE

Serine substitution for cysteine residues in levansucrase selectively abolishes levan forming activity

AUTHOR(S)
Senthilkumar, Velusamy; Busby, Stephen J.W.; Gunasekaran, Paramasamy
PUB. DATE
October 2003
SOURCE
Biotechnology Letters;Oct2003, Vol. 25 Issue 19, p1653
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Levansucrase is responsible for levan formation during sucrose fermentation of Zymomonas mobilis, and this decreases the efficiency of ethanol production. As thiol modifying agents decrease levan formation, a role for cysteine residues in levansucrase activity has been examined using derivatives of Z. mobilis levansucrase that carry serine substitutions of cysteine at positions 121, 151 or 244. These substitutions abolished the levan forming activity of levansucrase whilst only halving its activity in sucrose hydrolysis. Thus, polymerase and hydrolase activities of Z. mobilis levansucrase are separate and have different requirements for the enzyme's cysteine residues.
ACCESSION #
16762499

 

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