TITLE

Modified Technique for Measuring Platelet Aggregation

AUTHOR(S)
Kaplan, Alexander; Kaplan, Svetlana; Marcoe, Karen F.; Sauvage, Lester R.; Hammond, William P.
PUB. DATE
July 1997
SOURCE
Clinical & Applied Thrombosis/Hemostasis;Jul1997, Vol. 3 Issue 3, p196
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The turbidimetric method of Born is the current approach for assessing the aggregation behavior of platelets. It has been of modest practical value, due to difficulty in standardizing laboratory techniques for plasma preparation and inadequate quantification of the aggregation process. We report a new technique of sedimented platelet rich plasma (SPRP) preparation that reduces the irregularities caused by factors associated with procuring and preparing blood samples and permits a more flexible protocol for laboratory practice. We quantified results with a platelet aggregation score, which is calculated by multiplying the ratio of the height of the initial wave of aggregation to the maximum height of the aggregation pattern by the total area under the aggregation curve and by the ratio of the whole blood platelet count and the number of platelets in the sample. Comparative analysis of platelet aggregation scores (n = 95) obtained with both plasma preparation techniques using a paired t test demonstrated no statistical differences (t = 1.368. p = 0.174), To demonstrate the application of this modified method to evaluation of antiplatelet agents, the effects of aspirin and aspirin combined with citric acid on platelet aggregation were studied in vitro. The antiaggregatory effect of aspirin combined with citric acid was dependent on the pH and on their molar ratio, and was greater than the effect of aspirin alone. The SPRP protocol with platelet aggregation scoring methodology could be a valid alternative for measurement of the platelets" propensity to aggregate and the effect of antithrombotic treatments.
ACCESSION #
16709371

 

Related Articles

  • Influence of aspirin or heparin on platelet function and postoperative blood loss after coronary artery bypass surgery. Sirvinskas, Edmundas; Veikutiene, Audrone; Grybauskas, Pranas; Cimbolaityte, Jurate; Mongirdiene, Ausra; Veikutis, Vincentas; Raliene, Laima // Perfusion;Jan2006, Vol. 21 Issue 1, p61 

    The aim of the study was to assess the effect of aspirin or heparin pretreatment on platelet function and bleeding in the early postoperative period after coronary artery bypass grafting (CABG) surgery. Seventy-five male patients with coronary artery disease who underwent CABG with...

  • Citric Acid Enhances the Antithrombotic Effect of Aspirin in Many Aspirin-Resistant Subjects. Kaplan, Svetlana; Kaplan, Alexander; Marcoe, Karen F.; Hammond, William P.; Fisher, Lloyd D.; Sauvage, Lester R. // Clinical & Applied Thrombosis/Hemostasis;Jan1997, Vol. 3 Issue 1, p54 

    This study had three objectives: (1) to determine the frequency of high platelet aggregators in a consecutive series of 268 apparently healthy volunteers who presented to our Center; (2) to assess the inhibitory effect of aspirin (ASA) on these high aggregators; (3) to determine, in a...

  • A COMPARISON STUDY TO DETECT PLATELET SENSITIVITY TO ASPIRIN BY PLATELET AGGREGATION METHODS. Sanada, A. B.; Zaidah, A. Wan; Rosline, H.; Zurkurnai, Y.; M. H., Tee; Suhairi, I.; R., Madhavan // Malaysian Journal of Medical Sciences;Jan2007 Supplement, Vol. 14, p174 

    Introduction: Aspirin or acetylsalicylic acid is an anti-platelet agent used to prevent thromboembolic complications related to cardiovascular diseases. Unfortunately aspirin's anti-platelet effect may not be uniform in all patients. The aim of this study is to detect platelet sensitivity to...

  • Ten Years' Experience with the "Sticky Platelet Syndrome" Mammen, Eberhard F. // Clinical & Applied Thrombosis/Hemostasis;Winter1995, Vol. 1 Issue 1, p66 

    We describe a 10-year experience with a congenital platelet abnormality characterized by hyperaggregability with ADP and/or epinephrine. The syndrome has so far been identified in > 200 patients and their families. Clinical symptoms are transient or permanent arterial occlusions, thrombotic in...

  • aggregation, platelet. Peters, Michael // BMA A-Z Family Medical Encyclopedia;2004, p23 

    An encyclopedia entry for "platelet aggregation" is presented. It refers to the grouping of platelets that occurs when a blood vessel is damaged. It noted that platelet aggregation is the first step of blood clotting, helping close injured vessels. Drugs that help lessen platelet aggregation...

  • Should We RELOAD Patients on Long-term Clopidogrel? Boyle, Andrew J. // Clinical Cardiology Alert;Nov2008, Vol. 27 Issue 11, p81 

    The article discusses research being done on whether reloading with clopidogrel will improve platelet aggregation parameters. It references a study by J. P. Collet et al published in a 2008 issue of "Circulation." 166 patients who were stable on clopidogrel therapy served as subjects in this...

  • Heparin Therapy Leads to Platelet Activation and Prolongation of PFA 100 Closure Time.  // Journal of Cardiovascular Pharmacology & Therapeutics;Dec2005, Vol. 10 Issue 4, p273 

    The article focuses on a study that examined the in-vitro effects of unfractionated heparin on platelet function and PFA-100 testing. In the study platelet function analysis was done on a sample of whole blood using a PFA- 100. Platelet activation and aggregation was analyzed with and without...

  • New antiplatelet therapies in development. Price, Matthew J. // American Journal of Health-System Pharmacy;Jul2008 Supplement, Issue S5, pS11 

    Purpose. The efficacy and safety of new antiplatelet therapies, as well as antiplatelet therapies in development, are reviewed. Summary. Variability in the response to treatment with aspirin has been recognized for more than 40 years. Thienopyridine antiplatelet agents are now a standard...

  • Ticlopidine, Alka-Seltzer®, or a Combination of Citric Acid With Aspirin: Effects on Platelet Aggregation in Individuals With an Insufficient Response to Aspirin Alone. Kaplan, Svetlana; Kaplan, Alexander; Marcoe, Karen; Sauvage, Lester R. // Clinical & Applied Thrombosis/Hemostasis;Oct2000, Vol. 6 Issue 4, p222 

    Aspirin (ASA) does not effectively lower platelet aggregation in all people. The platelet aggregation (PA) score is an easily used clinical method for measuring the effect in individuals of antiplatelet medications. Fifteen apparently healthy subjects (2 men and 13 women), selected for their...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics