TITLE

Reversed-phase liquid chromatography and argentation chromatography of the minor capsaicinoids

AUTHOR(S)
Thompson, Robert Q.; Phinney, Karen W.; Sander, Lane C.; Welch, Michael J.
PUB. DATE
April 2005
SOURCE
Analytical & Bioanalytical Chemistry;Apr2005, Vol. 381 Issue 7, p1432
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An investigation of the liquid chromatography of the minor capsaicinoids in a commercial capsaicinoid mixture is reported. Twelve stationary phases including C8, C18, C30, phenyl, and cation-exchange chemistries were examined in combination with isocratic aqueous methanol and aqueous acetonitrile mobile phases. A phenyl stationary phase and aqueous acetonitrile mobile phase baseline-resolved 7 of 11 capsaicinoids, and selected ion chromatograms (LC-ESI-MS) demonstrated this was the most effective reversed-phase separation. Argentation chromatography with an alkyl or phenyl column and aqueous silver nitrate-methanol mobile phase revealed the presence of the 6-ene-8-methyl and 6-ene-9-methyl homocapsaicin isomers and the absence of 7-ene-9-methyl homocapsaicin. A mixed phenyl-cation-exchange stationary phase (charged with silver ion) enabled unique and useful separations of the capsaicinoids.
ACCESSION #
16700411

 

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