TITLE

Ventilator-induced heat shock protein 70 and cytokine mRNA expression in a model of lipopolysaccharide-induced lung inflammation

AUTHOR(S)
Vreugdenhil, Harriët A.; Haitsma, Jack J.; Jansen, Koos J.; Zijlstra, Jitske; Plötz, Frans B.; van Dijk, Jaap E.; Lachmann, Burkhard; van Vught, Hans; Heijnen, Cobi J.
PUB. DATE
June 2003
SOURCE
Intensive Care Medicine;Jun2003, Vol. 29 Issue 6, p915
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective. To investigate the effect of mechanical ventilation with no PEEP (ZEEP) and 4 cmH2O PEEP on heat shock protein 70 (HSP70) and pulmonary inflammatory cytokine expression in a model of lipopolysaccharide (LPS) induced lung inflammation. Design and setting. Prospective, randomized, experimental animal study. Subjects and interventions. We challenged 42 male Sprague-Dawley rats intratracheally with LPS. After 24 h the rats were randomly assigned to one of the ventilation strategies. Rats received either 4 h of mechanical ventilation with ZEEP or mechanical ventilation with 4 cmH2O PEEP. A nonventilated control group received LPS only. Lung pathology after LPS challenge was evaluated by histology to assess baseline lung injury. HSP70 and cytokine mRNA levels were measured in total lung homogenates. Results. PaO2 levels and lung histology revealed no deterioration after PEEP ventilation and severe deterioration after ZEEP ventilation. There was a significant higher expression of HSP70 and IL-1β mRNA in the lungs of the ZEEP group than in the PEEP group and nonventilated controls. In the ZEEP group high HSP70 levels were correlated inversely with low IL-1β mRNA and low IL-6 mRNA. Conclusions. We propose that HSP70 expression protects the lung against ventilator-induced lung injury by decreasing cytokine transcription in the lung.
ACCESSION #
16629436

 

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