TITLE

Calcitonin gene related peptide and N-procalcitonin modulate CD11b upregulation in lipopolysaccharide activated monocytes and neutrophils

AUTHOR(S)
Monneret, Guillaume; Arpin, Maud; Venet, Fabienne; Maghni, Karim; Debard, Anne-Lise; Pachot, Alexandre; Lepape, Alain; Bienvenu, Jacques
PUB. DATE
June 2003
SOURCE
Intensive Care Medicine;Jun2003, Vol. 29 Issue 6, p923
SOURCE TYPE
Academic Journal
DOC. TYPE
journal article
ABSTRACT
Objective: Circulating levels of calcitonin gene related peptide (CGRP) and calcitonin precursors, including procalcitonin (PCT) and its free aminopeptide N-procalcitonin (N-PCT), have been found dramatically increased in septic patients. PCT is known to attenuate the chemotaxis of monocytes in response to chemoattractants. This study examined whether CGRP and N-PCT modulate the LPS-induced expression of CD11b, which is one of the major integrins involved in monocyte and neutrophil chemotaxis during a response to microbial infections.Design and Setting: In vitro cell culture study in the immunology laboratory of a university hospital.Participants: Healthy volunteers.Measurements and Results: We assessed the effects of N-PCT and CGRP on CD11b expression on monocytes and neutrophils after LPS (2 ng/ml) or fMLP (10(-8) M) challenges. We used a human whole blood model, and measurements were made by flow cytometry. Both peptides in a dose-dependent manner decreased the LPS- and fMLP-induced rise in CD11b in monocytes and neutrophils. As these peptides are thought to act by raising cAMP, we also mimicked their effects with the use of rolipram and forskolin and found similar results.Conclusions: These findings are in line with recent studies demonstrating anti-inflammatory properties for this family of peptides. CGRP and calcitonin precursors may function as factors suppressing the propagation of inflammation through the inhibition of several processes involved during a response to a bacterial stimulus.
ACCESSION #
16629428

 

Related Articles

  • Leukocytes and neurogenic inflammation. Grant, Andrew // Inflammopharmacology;2002, Vol. 9 Issue 4, p403 

    Neurogenic inflammation is caused by the release of substance P and CGRP from sensory nerves, which stimulates microvascular plasma extravasation and vasodilatation. There is now an increasing body of evidence supporting a role for these neuropeptides in the control of the immune response, in...

  • Procalcitonin does not influence the surface expression of inflammatory receptors on whole blood leukocytes. Jørgensen, P. F.; Wang, J. E.; Solberg, R.; Thiemermann, C.; Foster, S. J.; Aasen, A. O.; Jørgensen, P F // Intensive Care Medicine;Feb2001, Vol. 27 Issue 2, p430 

    The plasma levels of procalcitonin (PCT) are increased in patients with severe bacterial infections. Its cellular origin and potential pathophysiological function in sepsis is, however, unclear. White blood cells have recently been described to express both PCT mRNA and protein. The aim of this...

  • Angiotensin-(1—7) stimulates water transport in rat inner medullary collecting duct: evidence for involvement of vasopressin V2 receptors. Magaldi, A. J.; Cesar, K. R.; e Araújo, M.; e Silva, A. C. Simões; Santos, R. A. S. // Pflugers Archiv European Journal of Physiology;Nov2003, Vol. 447 Issue 2, p223 

    The peptide angiotensin-(1–7) [Ang-(1–7)] is known to enhance water transport in rat inner medullary collecting duct (IMCD). The aim of this study was to determine the mechanism of the Ang-(1–7) effect on osmotic water permeability (Pf). Pf was measured in the normal rat...

  • Ocular hypotensive FP prostaglandin (PG) analogs: PG receptor subtype binding affinities and selectivities, and agonist potencies at FP and other PG receptors in cultured cells. Sharif, N. A.; Kelly, C. R.; Crider, J. Y.; Williams, G. W.; Xu, S. X. // Journal of Ocular Pharmacology & Therapeutics;Dec2003, Vol. 19 Issue 6, p501 

    Natural prostaglandins (PGs) such as PGD[sub 2], PGE[sub 2], PGF2[sub 2α], and PGI[sub 2] exhibited the highest affinity for their respective cognate receptors, but were the least selective agents when tested in receptor binding assays. Travoprost acid ([+]-fluprostenol) was the most...

  • Roles for thromboxane A2 and leukotrienes in endotoxin-induced acute renal failure. Badr, Kamal F.; Kelley, Vicki E.; Rennke, Helmut G.; Brenner, Barry M. // Kidney International;Oct1986, Vol. 30 Issue 4, p474 

    Bolus i.v. administration of 100 μg/kg of E. Coli lipopolysaccharide endotoxin (LPS) to adult male Munich-Wistar rats (N = 18) resulted in a progressive fall in RBF and GFR from 6.9 ± 0.2 SE and 1.1 ± 0.05 ml/min to minimal values at 50 minutes of 3.8 ± 0.4 and 0.32 ± 0.08 (P <...

  • Calcitonin Gene-Related Peptide (CGRP) Stimulates Purkinje Cell Dendrite Growth in Culture. D'Antoni, Simona; Zambusi, Laura; Codazzi, Franca; Zacchetti, Daniele; Grohovaz, Fabio; Provini, Luciano; Catania, Maria; Morara, Stefano // Neurochemical Research;Dec2010, Vol. 35 Issue 12, p2135 

    Previous reports described the transient expression during development of Calcitonin Gene-Related Peptide (CGRP) in rodent cerebellar climbing fibers and CGRP receptor in astrocytes. Here, mixed cerebellar cultures were used to analyze the effects of CGRP on Purkinje cells growth. Our results...

  • Topical Minoxidil in Alopecia Areata: No Effect on the Perifollicular Lymphoid Infiltration. Khoury, Emilio L.; Price, Vera H.; Abdel-Salam, Maha M.; Stern, Michael; Greenspan, John S. // Journal of Investigative Dermatology;Jul92, Vol. 99 Issue 1, p40 

    The therapeutic value of topical minoxidil in alopecia areata (AA) has been investigated in recent years, with variable results. Although the mechanism whereby minoxidil may stimulate hair regrowth in some cases of AA has not yet been elucidated, there have been reports of a decrease in the...

  • Evaluation of the cytogenetic damage induced by the antihypertensive drug nimodipine in human lymphocytes. Télez, Mercedes; Martínez, Begoña; Criado, Begoña; Ortega, Begoña; Peñagarikano, Olga; Flores, Piedad; Ortiz-Lastra, Eduardo; Arrieta, Isabel // Mutagenesis;Jul2001, Vol. 16 Issue 4, p345 

    The aim of this work was a study of the genotoxic potential of chronic long-term therapy with the antihypertensive drug nimodipine by measures of sister chromatid exchanges (SCE) and micronuclei (MN) in peripheral human lymphocytes of patients with long-term exposure to this drug. Peripheral...

  • Role of neurotrophin signalling in the differentiation of neurons from dorsal root ganglia and sympathetic ganglia. Ernsberger, Uwe // Cell & Tissue Research;Jun2009, Vol. 336 Issue 3, p349 

    Manipulation of neurotrophin (NT) signalling by administration or depletion of NTs, by transgenic overexpression or by deletion of genes coding for NTs and their receptors has demonstrated the importance of NT signalling for the survival and differentiation of neurons in sympathetic and dorsal...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics