Simple scheme for rapid three-dimensional orientation determination of the emission dipole of single molecules

Hohlbein, Johannes; Hübner, Christian G.
March 2005
Applied Physics Letters;3/21/2005, Vol. 86 Issue 12, p121104
Academic Journal
One of the unique features of single molecule absorption and emission is their anisotropy due to the well-defined transition dipole(s) for both processes allowing the determination of the molecule’s orientation. While polarization-resolved techniques are usually capable of detecting only a projection of the transition dipole, several methods have been proposed in order to determine the full three-dimensional orientation. Here, we report on a detection scheme that allows for a shot-noise limited determination of the emission dipole orientation utilizing an annular mirror, a polarizing beam splitter in conjunction with three detectors in a scanning confocal optical microscope.


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