TITLE

Application of coupled-column liquid chromatography combined with post-column photochemically induced fluorimetry derivatization and fluorescence detection to the determination of pyrethroid insecticides in vegetable samples

AUTHOR(S)
Parrilla Vázquez, P.; Gil García, M. D.; Barranco Martínez, D.; Martí Galera, M.
PUB. DATE
March 2005
SOURCE
Analytical & Bioanalytical Chemistry;Mar2005, Vol. 381 Issue 6, p1217
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This study reports the first application of coupled-column liquid chromatography-photochemically induced fluorimetry-fluorescence detection (LC-LC-PIF-FD), demonstrating its potential for the quantitative and selective detection of seven pyrethroids in vegetable samples such as cucumber, green bean, tomato and aubergine. An internal surface reversed-phase (ISRP) column coupled to a C18 column for analyte clean-up and determination were used, respectively. In comparison with a C18 column, the ISRP substantially improved the separation between analytes and interferences from the vegetable matrix. The limits of detection ranged from 0.01 to 0.22 µg kg-1 in the vegetable samples (equivalent to 0.01 and 0.13 µg L-1 in the extract injected), and limits of determination ranged from 0.56 to 8.33 µg kg-1 in the vegetable samples (equivalent to 0.34 and 5.00 µg L-1 in the extract injected). Samples were extracted into dichloromethane to yield mean recoveries at two levels of concentration between 72.8 and 110.0% in all cases. Relative standard deviations were lower than 11%.
ACCESSION #
16526169

 

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