Ultrasonic extraction followed by sulfuric acid silica gel cleanup for the determination of a-hexachlorocyclohexane enantiomers in biota samples

Rusong Zhao; Shaogang Chu; Rongbiao Zhao; Xiaobai Xu; Xiufen Liu
March 2005
Analytical & Bioanalytical Chemistry;Mar2005, Vol. 381 Issue 6, p1248
Academic Journal
Interest in the analysis of a-hexachlorocyclohexane (a-HCH) enantiomers as an alternative or complementary approach to elucidating isomer ratios of a/?-HCH has grown in recent years because it can provide useful information to evaluate the influence of different degradation and transformation processes. In this paper, a simple and rapid method for the determination of a-HCH enantiomers in biota samples is described. The method developed consists of ultrasonic extraction, sulfuric acid silica gel cleanup, solid-phase extraction (SPE) column fractionation, and final determination with chiral high-resolution gas chromatography. Ultrasonic extraction greatly shortens the extraction process time, and the sulfuric acid silica gel and SPE cleanup perfectly remove lipids and other interference compounds in the lipid-rich samples. The method is found to be simple, less time-consuming, and easy to operate, thus providing a useful alternative method to assess the enantioselective breakdown of a-HCH in biota system.


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