TITLE

Theoretical analysis of numerical aperture increasing lens microscopy

AUTHOR(S)
Ippolito, S. B.; Goldberg, B. B.; Ünlü, M. S.
PUB. DATE
March 2005
SOURCE
Journal of Applied Physics;3/1/2005, Vol. 97 Issue 5, p053105
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We present a detailed theoretical analysis and experimental results on a subsurface microscopy technique that significantly improves the light-gathering, resolving, and magnifying power of a conventional optical microscope. The numerical aperture increasing lens (NAIL) is a plano-convex lens placed on the planar surface of an object to enhance the amount of light coupled from subsurface structures within the object. In particular, a NAIL allows for the collection of otherwise inaccessible light at angles beyond the critical angle of the planar surface of the object. Therefore, the limit on numerical aperture increases from unity for conventional subsurface microscopy to the refractive index of the object for NAIL microscopy. Spherical aberration associated with conventional subsurface microscopy is also eliminated by the NAIL. Consequently, both the amount of light collected and diffraction-limited spatial resolution are improved beyond the limits of conventional subsurface microscopy. A theoretical optical model for imaging structures below the planar surface of an object, both with and without a NAIL, is presented. Experimental results demonstrating the predicted improvements in resolution of subsurface imaging are also presented.
ACCESSION #
16454294

 

Related Articles

  • Optics deserve attention. Titus, Jon // Test & Measurement World;Mar2005, Vol. 25 Issue 2, p19 

    Comments on the rejection of lens requirements for a new vision system. Determination of vision-system designers for lens specifications; Cause of the problems faced by system designers to larger imaging arrays.

  • Key Elements of Confocal Raman Microscopy for High-Resolution Imaging. Dieing, Thomas; Henrich, Marius; Breuninger, Sonja // Spectroscopy;Supplement 2014, p42 

    The sensitivity of a high-resolution Raman imaging system is crucial to the quality of the acquired information. The spectral and spatial resolutions are among the primary factors that influence the obtainable results. The limits of resolution are defined theoretically by the laws of physics,...

  • Wavefront corrected light sheet microscopy in turbid media. Dalgarno, H. I. C.; Cˇizˇmár, T.; Vettenburg, T.; Nylk, J.; Gunn-Moore, F. J.; Dholakia, K. // Applied Physics Letters;5/7/2012, Vol. 100 Issue 19, p191108 

    Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to...

  • An f-Theta Lens Design for Bio-Medical System: Laser Scanning Microarray Reader. C. Wen; K. Yuan; P. Chou; J. Huang; C. Lee // Optical & Quantum Electronics;Nov2005, Vol. 37 Issue 13-15, p1367 

    Laser scanning technology has put in microarray image system for its characteristic of non-contact measurement, high performance, and good sensitivities for the development of bio-technology. In the requirement of metrology, microarray biochip image has become more precisely and non-contact...

  • Webcam microscopics. Wentk, Richard // APC (Bauer Media Group);Jul2008, Vol. 28 Issue 7, p98 

    The article offers tips on how to attain clear images through using webcam. The author advises individuals to add a close-up focusing lens to the webcam to add a small magnification factor so that the webcam can focus correctly on the object. Once the focus set is ready, individuals are...

  • In vivo fluorescence imaging with high-resolution microlenses. Barretto, Robert P. J.; Messerschmidt, Bernhard; Schnitzer, Mark J. // Nature Methods;Jul2009, Vol. 6 Issue 7, p511 

    Micro-optics are increasingly used for minimally invasive in vivo imaging, in miniaturized microscopes and in lab-on-a-chip devices. Owing to optical aberrations and lower numerical apertures, a main class of microlens, gradient refractive index lenses, has not achieved resolution comparable to...

  • Multifunction test targets are used to test and calibrate imaging systems. Zuech, Nello // Vision Systems Design;Nov2008, Vol. 13 Issue 11, pD10 

    The article discusses the use of multi-function targets for performance evaluation and calibration of imaging systems. The entry notes that features of the Max Levy Autograph targets which perform the function of several targets, including standard resolution, distortion and depth-of-field...

  • SUPER RESOLUTION FROM MULTIPLE LOW RESOLUTION IMAGES. Manaila, Florin; Boiangiu, Costin-Anton; Bucur, Ion // Journal of Information Systems & Operations Management;Dec2014, Vol. 8 Issue 2, p100 

    Although the technology of optical instruments is constantly advancing, the capture of high resolution images is limited by both the shortcomings of the imaging devices and the law of physics (uncertainty principle applied onto photons or the wave-like theory of light). The current paper...

  • Computational ghost imaging of hot objects in long-wave infrared range. Hong-Chao Liu; Shuang Zhang // Applied Physics Letters;7/17/2017, Vol. 111 Issue 3, p1 

    Ghost imaging (GI) is an intriguing imaging modality to obtain the object information from the correlation calculations of spatial intensity fluctuations. In this letter, we report the computational GI of hot objects in the long-wave infrared range both in experiment and simulation. Without...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics