TITLE

The 13C-Urea Blood Test Accurately Detects Active Helicobacter pylori Infection: A United States, Multicenter Trial

AUTHOR(S)
Chey, W. D.; Murthy, U.; Toskes, P.; Carpenter, S.; Laine, L.
PUB. DATE
June 1999
SOURCE
American Journal of Gastroenterology;Jun1999, Vol. 94 Issue 6, p1522
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
OBJECTIVES: Current nonendoscopic tests for Helicobacter pylori include antibody tests and the urea breath test. After the administration of 13C-urea, serum bicarbonate measurement can identify those infected with H. pylori. In this study, our aims were to determine the accuracy of the urea blood test, and to compare the accuracy of the urea blood test with that of rapid urease testing of gastric biopsies. METHODS: This was a multicenter trial conducted at five sites within the U.S. Patients scheduled for endoscopy were recruited. During endoscopy, biopsies were obtained from the gastric body and antrum for histology and rapid urease testing. Patients underwent the urea blood test, which required the ingestion of 125 mg of 13-urea after endoscopy. Thirty minutes later, a 3-ml blood sample was obtained and later analyzed by mass spectrometry for 13C-bicarbonate. Performance characteristics for the urea blood test were calculated using the endoscopic biopsy tests as a gold standard. RESULTS: One hundred and twenty-one patients (54 infected) were enrolled. The urea blood test yielded sensitivity of 89%, specificity of 96%, positive predictive value of 94%, negative predictive value of 91%, and accuracy of 93% using histology as a gold standard. There was no difference between results obtained with the urea blood test and rapid urease testing of gastric biopsies. CONCLUSIONS: The urea blood test accurately identified active H. pylori infection. The performance characteristics of this nonendoscopic test were similar to those of endoscopic rapid urease testing.
ACCESSION #
16444807

 

Related Articles

  • Genotyping of Helicobacter pylori in paraffin-embedded gastric biopsy specimens: relation to histological parameters and effects on therapy. Scholte, Ger H.A.; van Doorn, Leen-Jan; Cats, Annemieke; Bloemena, Elisabeth; Lindeman, Jan; Quint, Wim G.V.; Meuwissen, Stefan G.M.; Kuipers, Ernst J. // American Journal of Gastroenterology;Jul2002, Vol. 97 Issue 7, p1687 

    OBJECTIVES:Colonization with Helicobacter pylori can lead to GI disease. Bacterial genotypes and host factors, such as acid production, can influence the progress of disease. We investigated H. pylori genotypes and histological parameters in the same paraffin-embedded gastric biopsy...

  • Evaluation of a New Urease Reagent Strip for Detection of Helicobacter pylori in Gastric Biopsy Specimens. Rogge, James D.; Mahoney, Stephen J.; Wagner, David R.; Carrico, Robert J.; Boguslaski, Robert C.; Genta, Robert M.; Glowinski, Elizabeth A. // American Journal of Gastroenterology;Nov1995, Vol. 90 Issue 11, p1965 

    Objectives: Determine sensitivity and specificity of a new urease reagent strip (URS) test for detection of Helicobacter pylori in gastric biopsy specimens. Methods: Six paired biopsy specimens were obtained from the greater curvature of the distal antrum, the lesser curvature near the...

  • Campylobacter pylori in Patients with Dyspeptic Symptoms and Endoscopic Evidence of Erosion(s). Elta, Grace H.; Murphy, Rosanne; Behler, Elizabeth M.; Barnett, Jeffrey L.; Nostrant, Timothy T.; Kern, Scott; Appelman, Henry // American Journal of Gastroenterology;Jun1989, Vol. 84 Issue 6, p643 

    The relationship between Campylobacter pylori (CP), histologic gastritis, and dyspeptic symptoms is becoming gradually clearer, but there is still a lack of knowledge of the natural history of treated or untreated gastritis. We examined serial biopsies from the gastric fundus, body, and...

  • Helicobacter heilmanniiinfection in a child after successful eradication ofHelicobacter pylori: case report and review of literature. Kato, Seiichi; Ozawa, Kyoko; Sekine, Hitoshi; Ohyauchi, Mikio; Shimosegawa, Tooru; Minoura, Takanori; Iinuma, Kazuie // Journal of Gastroenterology;2005, Vol. 40 Issue 1, p94 

    An 11-year-old boy withHelicobacter pylori-associated duodenal ulcer was successfully treated with a combination of lansoprazole, amoxicillin, and clarithromycin. Endoscopy and gastric biopsies were repeated 2 and 12 months later, showing ulcer healing and eradication ofH. pylori. However, a...

  • Biopsy Sites Suitable for the Diagnosis of Helicobacter pylori Infection and the Assessment of the Extent of Atrophic Gastritis. Satoh, Kiichi; Kimura, Ken; Taniguchi, Yushi; Kihira, Ken; Takimoto, Takuya; Saifuku, Koji; Kawata, Hiroshi; Tokumaru, Kenkichi; Kojima, Toshichika; Seki, Masaru; Ido, Kenichi; Fujioka, Toshio // American Journal of Gastroenterology;Apr1998, Vol. 93 Issue 4, p569 

    Objectives: We performed this study to determine which biopsy sites in the stomach are suitable for the diagnosis of Helicobacter pylori infection and the assessment of the extent of atrophic gastritis. Methods: Endoscopy was performed in 76 H. pylori-positive patients with...

  • Clinical Utility and Cost Effectiveness of Helicobacter pylori Testing for Patients with Duodenal and Gastric Ulcers. Greenberg, Paul D.; Koch, Johannes; Cello, John P. // American Journal of Gastroenterology;Feb1996, Vol. 91 Issue 2, p228 

    Objective: Current consensus guidelines recommend that all patients demonstrating either a gastric or duodenal ulcer be tested for Helicobacter pylori, the most common cause of ulcers. We determined the clinical utility and cost effectiveness of H. pylori testing in patients...

  • Microdose 14C-Urea Breath Test Offers Diagnosis of Helicobacter pylori in 10 Minutes. Peura, David A.; Pambianco, Daniel J.; Dye, Kevin R.; Lind, Christopher D.; Frierson, Henry F.; Hoffman, Susie R.; Combs, Matthew J.; Guilfoyle, Elizabeth; Marshall, Barry J. // American Journal of Gastroenterology;Feb1996, Vol. 91 Issue 2, p233 

    Objectives: The urea breath test diagnoses Helicobacter pylori infection of the stomach by identifying the urease enzyme activity of the bacterium. In this "microdose" version of the test, 1 µCi 14C-urea is given orally in a capsule. Our objectives were: 1) to evaluate a microdose...

  • Helicobacter pylori Prevalence in Acquired Immunodeficiency Syndrome. Marano Jr., Benjamin J.; Smith, Fred; Bonanno, Charles A. // American Journal of Gastroenterology;May1993, Vol. 88 Issue 5, p687 

    Helicobacter pylori is consistently reported with prevalence in HlV-negative patients with chronic gastritis and active ulcer disease. This study is an evaluation of the prevalence of H. pylori in AIDS patients, and the association with chronic gastritis, erosions, and ulcer disease....

  • Histologic Demonstration Of Helicobacter Pylori In Gastric Biopsies: Which Is The Best Staining Method? Kacar, F�ruzan; �ulhaci, Nil; Y�kselen, Vahit; Meteo&gcaron;lu, &Iring;brahim; Dikicio&gcaron;lu, Emel; Levi, Edi // Internet Journal of Pathology;2004, Vol. 3 Issue 1, p1 

    Aims: We have investigated the specificity and sensitivity of different histochemical and immunohistochemical methods for the detection of Helicobacter pylori (HP) on tissue sections of gastric biopsies. In addition, interobserver agreement of different staining methods was also evaluated in...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics