TITLE

Microdose 14C-Urea Breath Test Offers Diagnosis of Helicobacter pylori in 10 Minutes

AUTHOR(S)
Peura, David A.; Pambianco, Daniel J.; Dye, Kevin R.; Lind, Christopher D.; Frierson, Henry F.; Hoffman, Susie R.; Combs, Matthew J.; Guilfoyle, Elizabeth; Marshall, Barry J.
PUB. DATE
February 1996
SOURCE
American Journal of Gastroenterology;Feb1996, Vol. 91 Issue 2, p233
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objectives: The urea breath test diagnoses Helicobacter pylori infection of the stomach by identifying the urease enzyme activity of the bacterium. In this "microdose" version of the test, 1 µCi 14C-urea is given orally in a capsule. Our objectives were: 1) to evaluate a microdose 14C-urea breath test capsule in a gastroenterology outpatient setting, 2) to determine the diagnostic ranges of the 14C-urea breath test for HP-positive and HP-negative patients, 3) to define the sensitivity and specificity of the test, and 4) to see whether breath sample results changed when they were mailed to a remote site for analysis. Methods: In a prospective blinded study, we breath-tested 200 fasted patients before elective outpatient endoscopy. At endoscopy, two gastric biopsy samples were taken and were examined for curved organisms; a third biopsy specimen was evaluated with a rapid urease test (CLOtest). Breath samples were mailed in aluminized balloons to a testing laboratory. Results: Using a single breath sample collected at 10 min, with ≥200 dpm as positive, the breath test correctly classified 63 of 65 HP-positive patients (sensitivity 97%, CI 89-99%), and 128 of 135 HP-negative patients (specificity 95%, CI 90-98%). Radiation exposure from the test equated to natural background received in 1 day. No adverse events were caused by the breath test. Conclusions: The 14C-urea capsule breath test (PYtest) is a convenient noninvasive test for the detection of gastric H. pylori infection. Accuracy is equivalent to invasive methods such as histology. Results can be obtained within 15 min if a counting instrument is nearby, or breath samples can be mailed to a testing laboratory for analysis.
ACCESSION #
16440144

 

Related Articles

  • Accuracy of breath tests using low doses of 13C-urea to diagnose Helocobacter pylori infection: a randomized controlled trial. Gatta, L.; Ricci, C.; Tampieri, A.; Oshborn, J.; Ferna, F.; Bernabucci, V.; Vaira, D. // Gut;Apr2006, Vol. 55 Issue 4, p457 

    Background: The 13C-urea breath test (UBT) for detecting Helicobacter pylon infection is ion-invasive method based on the organism's urease activity. Since its first description, the method has been extensively modified. However, only the dose of 13C-urea and the measurement equipment are...

  • Posttreatment13C-Urea Breath Test Is Predictive of Antimicrobial Resistance toH. pyloriAfter Failed Therapy. Kao, Ai-Wen; Cheng, Hsiu-Chi; Sheu, Bor-Shyang; Lin, Ching-Yih; Sheu, Ming-Jen; Yang, Hsiao-Bai; Wu, Jiunn-Jong // JGIM: Journal of General Internal Medicine;Feb2005, Vol. 20 Issue 2, p139 

    We tested whether a13C-urea breath test can predict antimicrobial resistance ofHelicobacter pylori(H. pylori).Seventy patients who had failed triple eradication therapy and 108 untreatedH. pylori-infected patients were given a13C-urea breath test, endoscopy for culture ofH. pylori, and...

  • The Impact of Delaying Gastric Emptying by Either Meal Substrate or Drug on the [13C]-Urea Breath Test. Casellas, F.; Lopez, J.; Borruel, N.; Saperas, E.; Vergara, M.; De Torres, I.; Armengol, J. R.; Malagelada, J.-R. // American Journal of Gastroenterology;Feb1999, Vol. 94 Issue 2, p369 

    Objective: Optimal [13C]-urea breath test (UBT) conditions for diagnosis of Helicobacter pylori infection are still being fine-tuned. In the present study we investigated the impact of delaying gastric emptying by different meal substrates or L-DOPA, a drug known to induce gastric stasis, on the...

  • Helicobacter pylori breath tests. Berger, Abi // BMJ: British Medical Journal (International Edition);5/25/2002, Vol. 324 Issue 7348, p1263 

    Details how diagnostic breath tests for Helicobacter pylori work. How the baseline sample is taken with a sachet of orange juice or citric acid; How the post-dose sample is taken after a drink containing [sup13]C enriched urea; Association of ulcers with H pylori infections.

  • Biokinetics and radiation doses for carbon-14 urea in adults and children undergoing the Helicobacter pylori breath test. Leide-Svegborn, Sigrid; Stenström, Kristina; Olofsson, Mikael; Mattsson, Sören; Nilsson, Lars-Erik; Nosslin, Bertil; Pau, Kinga; Johansson, Lennart; Erlandsson, Bengt; Hellborg, Ragnar; Skog, Göran // European Journal of Nuclear Medicine;1999, Vol. 26 Issue 6, p573 

    Abstract. The long-term biokinetics and dosimetry of carbon-14 were studied in nine adults and eight children undergoing carbon-14 urea breath test for Helicobacter priori (HP) infection. The elimination of 14C via exhaled air and urine was measured with the liquid scintillation counting...

  • Clinical studies of 13C-urea breath test in Japan. Kato, Mototsugu; Asaka, Mashiro; Ohaka, Shuichi; Toyota, Takavosii // Journal of Gastroenterology;1998 Supplement 10, Vol. 33, p36 

    The urea breath test (UBT) using isotope carbon is a non-invasive method for diagnosing hr. pylori infection. In Japan, only 13C-UBT using a stable isotope is available, since the clinical use of radioactive isotope 14C-compounds is prohibited. Since there is no worldwide standard protocol for...

  • Clinical importance of baseline measurement in [sup 13]C-urea breath test. Yang, J.C.; Kuo, Y.N.; Lee, S.C.; Yang, T.H.; Twu, S.J.; Wang, T.H. // Gut;Sep2002 Supplement 2, Vol. 51, pA107 

    Introduction: Some investigators have suggested that the H. pylori infective status can be determined from a single 30-min post-dose breath sample of the [sup 13]C-urea breath test ([sup 13]C-UBT). The aim of this study was to evaluate the clinical importance of baseline measurement in [sup...

  • Validation of [sup 13]C-urea breath test for the initial diagnosis of Helicobacter pylori infection and for the confirmation of its eradication after treatment. Gisbert, J.P.; Ducons, J.; Gomollon, F.; Dominguez-Muñoz, E.; Borda, F.; Miño, G.; Jimenez, I.; Vazquez, M.A.; Santolaria, S.; Gallego, S.; Iglesias, J.; Pastor, G.; Hervas, A.; Pajares, J.M. // Gut;Sep2002 Supplement 2, Vol. 51, pA109 

    Aim: [sup 13]C-urea breath test (UBT) is a diagnostic method widely used in several countries, but its accuracy has not been properly confirmed in a Clinical Trial in our country. Our aim was to evaluate the performance of UBT for both the initial diagnosis of H. pylori and the confirmation of...

  • Stool antigen test for non-invasive post-treatment assessment of Helicobater pylori infection: A comparative study between immunoenzymatic and immunochromatographic tests. Zanetti, M.V.; Vendramin, R.; Pieramico, O. // Gut;Sep2002 Supplement 2, Vol. 51, pA111 

    It is known that Helicobacter pylori (HP) infection may be detected by invasive and/or not invasive techniques. Urea breath-test (UBT) and Stool test (ST) are the most accurate, reliable and widely used non-invasive techniques. After the enzyme immunoassay using polyclonal antibodies against HP...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics