Valdivia, Enrique; Olson, Ward; Simon, Stacy
October 1961
Angiology;Oct1961, Vol. 12 Issue 10, p462
Academic Journal
A direct illumination method is described that permits observation of the pulmonary vessels on the external surface of the lung. The preparation requires general anesthesia, endoctracheal intubation and open thorax. Artificial ventilation of the lung is recommended to prevent carbon dioxide accumulation and alterations of the pulmonary pressure. Carbon dioxide accumulation and rise in the pulmonary pressure are seen in dogs submitted to �still lung� preparations for observation of the pulmonary vessels. Our method utilizes local immobilization of the lung with a round cover glass held in situ with a plastic disc. Illumination is obtained with an incident light illuminator as commercially made for metallurgic microscopes. When photography or cinematographic records are taken, the illuminator is replaced with a larger reflection box, and carbon are light is used as a source of light. Two groups of guinea pigs have been studied with this method, one maintained at sea level and the other exposed to simulated high altitude. The capillaries lining the air spaces of animals kept at high altitude are longer and wider.


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