Miller, Walter R.; Willson, John T.; Eliot, Theodore S.
October 1959
Angiology;Oct1959, Vol. 10 Issue 5, p375
Academic Journal
The early effects of muscle contusion trauma in dogs were investigated with respect to the coagulation mechanism, blood elements, plasma proteins, and microcirculatory changes. A significant decrease in clotting time occurred, whereas the prothrombin time, prothrombin consumption, platelet count, and clot retraction remained unchanged. The platelet thromboplastic factor was unaltered 3½ hours after trauma, but at 9½ hours its activity was decreased. Fibrinogen, as determined by the tyrosine method, revealed a progressive increase from the control to the 3½ - and 9½ -hour posttrauma samples. We attribute the hypercoagulability to either an increase in concentration of the prothrombin complex, an acceleration of thromboplastic activity (possibly due to tissue thromboplastic substance), or a decrease in anticoagulant activity. The erthrocyte count and hematocrit were very significantly increased, whereas the sedimentation rate was decreased. The riticulocyte count was unchanged 3½ hours after trauma but revealed an increase in the 9½ -hour posttrauma sample. The total leukocyte count showed a highly significant increase in the 9½ -hour posttrauma blood. The differential leukocyte counts were similar to those found in response to stress-lymphopenia, eosinopenia, and neutrophilia. Erythrocyte fragility was unchanged throughout the experiment. Electrophoretic analysis of the 3½ -hour posttrauma plasma sample revealed a significant and disproportionate increase in total protein, α2, γ, α3, and albumin. The changes in the four other components were insignificant. The lack of uniformity in protein concentration of the individual components is the result of both a selective addition of proteins to the plasma and a selective loss of proteins. Approximately 3 hours after contusion the mesenteric circulation revealed an intravascular agglutination of erythrocytes, reduction of flow rate, leukocytes adhering to venule walls, apparent hemoconcentration, and constriction of arterioles. These changes, with the exception of hemoconcentration and arteriolar constriction, became progressively more pronounced until the animal was killed 10½ hours after traumatization.


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