TITLE

AN EXPERIMENTAL METHOD FOR QUANTITATIVE EVALUATION OF THROMBOLYSIS: EFFECT OF CA-7, A PROTEASE FROM ASPERGILLUS ORYZAE

AUTHOR(S)
Giering, John E.; Truant, Aldo P.
PUB. DATE
September 1966
SOURCE
Angiology;Sep1966, Vol. 17 Issue 9, p661
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A quantitative method is described which is based upon the insertion into the external jugular vein of a preformed, weighed blood clot formed and retracted around a special framework. The frame firmly supports and confines the clot in a fixed position within the vein without completely impeding the free flow of blood. Following administration of thrombolytic agent, the clot can be recovered with ease from the vein, reweighed, and dissolution determined accurately. Futher, since it is not necessary to sacrifice or severely traumatize the animal in order to remove the clot, observations can be extended through the post-treatment period. Simplicity of the method provides opportunity for rapid screening to determine efficacy of new thromolytic agents and provides an additional experimental model for evaluation of these agents. The preliminary assessment of thrombolytic activity of CA-7, a purified proteolytic enzyme derived from the growth of Aspergillus oryzae, has been presented as an illustration of methodological utility and to provide supportive evidence to that already reported by others that this enzyme is able to lyse clots effectively in vivo providing dosage is rigidly controlled.
ACCESSION #
16378067

 

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