TITLE

Towards a fast-responding, label-free electrochemical DNA biosensor

AUTHOR(S)
Mir, M.; Katakis, I.
PUB. DATE
March 2005
SOURCE
Analytical & Bioanalytical Chemistry;Mar2005, Vol. 381 Issue 5, p1033
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
DNA sensors and sensor arrays (biochips) have become an important tool in molecular biology and biotechnology in recent years. For low-throughput, easy-to-use devices it is desirable that they be of low cost, reagentless, and label-free. Displacement sensors with electrochemical detection offer these advantages, and therefore the development of such a detection principle is show in this work. An HRP-labeled oligonucleotide was sub-optimally pre-hybridized with a capture probe and was displaced upon introduction of the fully complementary probe target, producing a decrease in signal that was proportional to the sample concentration. This detection scheme has been demonstrated colorimetrically and electrochemically, obtaining a total signal displacement of 55% only 5 min after introduction of the sample.
ACCESSION #
16371463

 

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