TITLE

Photometric detection of cyclodextrins in liquid chromatography by using iodine generated electrochemically in-situ

AUTHOR(S)
Hiroi, Toshiya; Jin, Jiye; Takeuchi, Toyohide
PUB. DATE
March 2005
SOURCE
Analytical & Bioanalytical Chemistry;Mar2005, Vol. 381 Issue 5, p1089
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A method has been developed for photometric detection of cyclodextrins (CD) in liquid chromatography using iodine (I2) generated electrochemically in-situ. Iodide ion in the mobile phase was electrochemically oxidized to I2 which was subsequently reacted with I-, in an electrochemical flow cell, forming I. The absorbance of Iwas found to be greatly enhanced when CD were present in the mobile phase. The absorbance enhancement was caused by the change in the mole fraction of I, because of the inclusion reaction of Iwith CD. On the basis of this phenomenon, CD were detected by means of a photodiode-array UV-visible detector positioned downstream of the electrochemical flow cell. The signals were found to be linearly dependent on CD concentration. Because the formation constants of Iwith CD decrease in the order a-CD>ß-CD>?-CD, a-CD was most detectable by the method. Detection limits were 1.0 µmol L-1 for a-CD, 65 µmol L-1 for monoG1-ß-CD, 100 µmol L-1 for ß-CD, and 200 µmol L-1 for ?-CD.
ACCESSION #
16371459

 

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