TITLE

Lichen-based biosensor for the determination of benzene and 2-chlorophenol: microcalorimetric and amperometric investigations

AUTHOR(S)
Antonelli, Marta Letizia; Campanella, Luigi; Ercole, Patrizia
PUB. DATE
March 2005
SOURCE
Analytical & Bioanalytical Chemistry;Mar2005, Vol. 381 Issue 5, p1041
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Preliminary microcalorimetric studies have been performed to analyse the response of a whole epiphytic lichen tissue (Evernia prunastri) to 2-chlorophenol (2Cl-?), a pollutant of oil mill waste-water, in order to evaluate whether the tissue might be used to assess the toxic characteristics of polluted waters. The obtained results (lichen viability expressed in hours, enthalpy variations for the 2Cl-?/lichen interactions) were used to create a lichen-based biosensor that uses an amperometric oxygen electrode (a Clark electrode) as a transducer. The lichen catalyses aromatic ring cleavage (via pyrocatechase enzymes present in the lichen), and transforms aromatic substances like 2Cl-? into muconic acid (C6H6O4). Following a full electroanalytical characterisation, the performance of the proposed lichen biosensor was compared to that of a biosensor based onPseudomonas putidacells, which was originally constructed to monitor benzene in different matrices (water, air, petrol and oil) and was tested in our laboratory previously.
ACCESSION #
16371444

 

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