Stefanini, Mario; Marin, Hector M.; Soardi, Franco; Mossa, Andrea
June 1962
Angiology;Jun1962, Vol. 13 Issue 6, p254
Academic Journal
This article examines the fibrinolytic activity and the influence on the blood clotting mechanism of Aspergillin O in vivo and compares its activity with that of trypsin under uniform experimental conditions. Aspergillin O, a purified extract from filtrates of cultures of Aspergillus oryzae B-2173, exhibits proteolytic, fibrinolytic and esterase activity in vitro. Experiments in vivo have indicated that the infusion of 10 mg of aspergillin O per kg of body weight in the general circulation of dogs reestablishes the blood flow through venous segments 48 hours after their occlusion by thrombi produced with sodium morrhuate. Aspergillin O was prepared from liquid cultures of Aspergillus oryzae B-2173. All preparations were lyophilized and stored at -20 degrees celsius until used. The comparative activity in vitro of trypsin and aspergillin O was established in terms of their ability to digest casein by using the method of Kunitz and a series of dilutions of the materials being investigated.


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