INCIDENT DARK-FIELD ILLUMINATION: A NEW METHOD FOR MICROCIRCULATORY STUDY
- Fiber shines light on many industries. Clarkson, Charles // Laser Focus World;Aug2000, Vol. 36 Issue 8, p197
Highlights the applications of fiber optic illumination systems in microscopy and machine-vision in industries. Parameters optimized during illumination system design; Benefits of fiber optic illumination; Components of a fiber optic illumination system.
- Microvascular engineering in perfusion culture: immunohistochemistry and CLSM findings. Frerich, Bernhard; Zückmantel, Kerstin; Hemprich, Alexander // Head & Face Medicine;2006, Vol. 2, p26
Background: One of the most challenging problems in tissue engineering is the establishment of vascular supply. A possible approach might be the engineering of microvasculature in vitro and the supply by engineered feeder vessels. Methods: An in vitro model for a small-diameter vessel was...
- 4D brain signaling. Marandi, Nima; Konnerth, Arthur // Nature Methods;Jan2007, Vol. 4 Issue 1, p19
The article focuses on the method of time-resolved two-photon volume imaging with cellular resolution allows for the first time a comprehensive analysis of cortical microcircuits in vivo. This advance has benefited from the progress in the field of two-photon-excited fluorescence laser scanning...
- Light Characteristics Limit Optical Quality. Masi, C.G. // Test & Measurement World;Jun2000, Vol. 20 Issue 8, p77
Considers limitations of the third-order theory that governs Seidel aberrations, nonlinear effects of light rays that exist too far from the optical axis of microscope lenses. Wavelengths of light in an image; Degradation of microscope performance through chromatic aberration and diffraction.
- dark field microscopy:. Schlegel, Rolf H. J. // Encyclopedic Dictionary of Plant Breeding & Related Subjects;2003, p119
A definition of the term "dark field microscopy" is presented. It refers to a microscope designed to black out the entering center light rays and to direct the peripheral rays against the object from the side. The result of such situation is given,
- Multibeam interferometric illumination as the primary source of resolution in optical microscopy. Ryu, J.; Hong, S. S.; Horn, B. K. P.; Freeman, D. M.; Mermelstein, M. S. // Applied Physics Letters;4/24/2006, Vol. 88 Issue 17, p171112
High-resolution images of a fluorescent target were obtained using a low-resolution optical detector by illuminating the target with interference patterns produced with 31 coherent beams. The beams were arranged in a cone with 78Â° half angle to produce illumination patterns consistent with a...
- Phase-gradient microscopy in thick tissue with oblique back-illumination. Ford, Tim N; Chu, Kengyeh K; Mertz, Jerome // Nature Methods;Dec2012, Vol. 9 Issue 12, p1195
Phase-contrast techniques, such as differential interference contrast microscopy, are widely used to obtain morphological images of unstained biological samples. The transillumination geometry required for these techniques restricts their application to thin samples. We introduce oblique...
- Deep tissue two-photon microscopy. Helmchen, Fritjof; Denk, Winfried // Nature Methods;Dec2005, Vol. 2 Issue 12, p932
With few exceptions biological tissues strongly scatter light, making high-resolution deep imaging impossible for traditionalâ€”including confocalâ€”fluorescence microscopy. Nonlinear optical microscopy, in particular two photonâ€“excited fluorescence microscopy, has overcome this...
- Structural characteristics of rootÂ–fungal interactions for five ericaceous species in eastern Canada. Massicotte, H. B.; Melville, L. H.; Peterson, R. L. // Canadian Journal of Botany;Aug2005, Vol. 83 Issue 8, p1057
A combination of light microscopy (including differential interference contrast) and laser scanning confocal microscopy was used to document the colonization patterns of epidermal cells and details of intracellular hyphal complexes of five native ericaceous hosts: Vaccinium oxycoccos L. (bog...