DNA separation with low-viscosity sieving matrix on microfabricated polycarbonate microfluidic chips

Mei-Ying Ye; Xue-Feng Yin; Zhao-Lun Fang
February 2005
Analytical & Bioanalytical Chemistry;Feb2005, Vol. 381 Issue 4, p820
Academic Journal
Microfluidic devices have been fabricated on polycarbonate (PC) substrates by use of a hot embossing method using a silicon master template. By adding auxiliary lines around the functional channel on the silicon master, its lifetime was significantly prolonged and the bonding strength of the PC cover plate to the microfluidic chip was greatly improved. More than 300 polycarbonate microfluidic chips have been replicated with the same silicon mold. CE separation of F X-174/HaeIII DNA restriction fragments, with high resolution efficiency and good reproducibility, was achieved on these devices using the low-viscosity sieving matrix HPMC-50. Temperature was found to have a significant effect on separation efficiency.


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