TITLE

High-resolution Hadamard transform microscope fluorescence imaging: quantifying the DNA content in single cells

AUTHOR(S)
Hongwu Tang; Meina Luo; Yongpan Xiong; Guanquan Chen
PUB. DATE
February 2005
SOURCE
Analytical & Bioanalytical Chemistry;Feb2005, Vol. 381 Issue 4, p901
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This study presents a novel miniaturized Hadamard transform fluorescence imaging microscope, by combining a conventional fluorescence microscope with Hadamard transform multiplexing encoding using a one-dimensional movable mask to realize spatial resolution and a linear CCD for multichannel detection. The microscope can provide high-resolution automatically-generated 0-255 gray level HT images for morphological analysis and visualization of a single cell, and normalized HT images for cellular quantitative measurements. The microscope’s imaging capability was applied to measure the DNA content in human lymphocyte, chicken erythrocyte and eel erythrocyte, and a comparative study was performed. The results show that the calibrated DNA content in a chicken erythrocyte is 2.32 pg when human lymphocyte is used as the standard, and eel erythrocyte may be a potentially reliable and novel standard for determining DNA contents in other species because it has a stable DNA value of 2.06 pg, with a CV of 4.3% when 20 eel erythrocytes are measured. The results also demonstrate that the HT imaging microscope should be valuable in the fields of medicine and cell biology.
ACCESSION #
16312062

 

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