TITLE

Calibration of NO sensors for in-vivo voltammetry: laboratory synthesis of NO and the use of UV-visible spectroscopy for determining stock concentrations

AUTHOR(S)
Brown, Finbar O.; Finnerty, Niall J.; Bolger, Fiachra B.; Millar, Julian; Lowry, John P.
PUB. DATE
February 2005
SOURCE
Analytical & Bioanalytical Chemistry;Feb2005, Vol. 381 Issue 4, p964
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The increasing scientific interest in nitric oxide (NO) necessitates the development of novel and simple methods of synthesising NO on a laboratory scale. In this study we have refined and developed a method of NO synthesis, using the neutral Griess reagent, which is inexpensive, simple to perform, and provides a reliable method of generating NO gas for in-vivo sensor calibration. The concentration of the generated NO stock solution was determined using UV-visible spectroscopy to be 0.28±0.01 mmol L-1. The level of NOcontaminant, also determined using spectroscopy, was found to be 0.67±0.21 mmol L-1. However, this is not sufficient to cause any considerable increase in oxidation current when the NO stock solution is used for electrochemical sensor calibration over physiologically relevant concentrations; the NO sensitivity of bare Pt-disk electrodes operating at +900 mV (vs. SCE) was 1.08 nA µmol-1 L, while that for NOwas 5.9×10-3 nA µmol-1 L. The stability of the NO stock solution was also monitored for up to 2 h after synthesis and 30 min was found to be the time limit within which calibrations should be performed.
ACCESSION #
16312049

 

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