Calibration of NO sensors for in-vivo voltammetry: laboratory synthesis of NO and the use of UV-visible spectroscopy for determining stock concentrations

Brown, Finbar O.; Finnerty, Niall J.; Bolger, Fiachra B.; Millar, Julian; Lowry, John P.
February 2005
Analytical & Bioanalytical Chemistry;Feb2005, Vol. 381 Issue 4, p964
Academic Journal
The increasing scientific interest in nitric oxide (NO) necessitates the development of novel and simple methods of synthesising NO on a laboratory scale. In this study we have refined and developed a method of NO synthesis, using the neutral Griess reagent, which is inexpensive, simple to perform, and provides a reliable method of generating NO gas for in-vivo sensor calibration. The concentration of the generated NO stock solution was determined using UV-visible spectroscopy to be 0.28±0.01 mmol L-1. The level of NOcontaminant, also determined using spectroscopy, was found to be 0.67±0.21 mmol L-1. However, this is not sufficient to cause any considerable increase in oxidation current when the NO stock solution is used for electrochemical sensor calibration over physiologically relevant concentrations; the NO sensitivity of bare Pt-disk electrodes operating at +900 mV (vs. SCE) was 1.08 nA µmol-1 L, while that for NOwas 5.9×10-3 nA µmol-1 L. The stability of the NO stock solution was also monitored for up to 2 h after synthesis and 30 min was found to be the time limit within which calibrations should be performed.


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