Characterization of the Cell-Bound Polysaccharides of Bifidobacterium adolescentis 94 BIM

Novik, G.I.; Astapovich, N.I.; Kubler, J.; Gamian, A.
March 2002
Microbiology (00262617);Mar2002, Vol. 71 Issue 2, p173
Academic Journal
The cell-bound polysaccharides (PSs) of Bifidobacterium adolescentis 94 BIM were stripped from the cell surface with 2% sodium dodecyl sulfate (SDS), 1.5% Cetavlon, and 1% Triton X-100 and purified by precipitation with 5 volumes of ethanol. According to the extraction conditions used, the polysaccharide preparations were designated as PS-SDS-6°C, PS-SDS-100°C, PS-Cet, and PS-Trit. The gel-permeation chromatography of the first two preparations with the use of a Bio-Gel P-10 column and 1% acetic acid as the eluant yielded two peaks, F1 and F2, which contained carbohydrates and no phosphorus. All polysaccharides were primarily composed of glucose and galactose. The polysaccharides PS-Cet and PS-Trit were found to be branched and contain glucose residues at the terminal position, position 4, and position 6, and galactose residue at position 3. PS-SDS-6°C has a glucose residue at position 4.


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