TITLE

Electrochemical characteristics of a platinum electrode modified with a matrix of polyvinyl butyral and colloidal Ag containing immobilized horseradish peroxidase

AUTHOR(S)
Ruo Yuan; Yan Liu; Qun-Fang Li; Ya-Qin Chai; Chang-Li Mo; Xia Zhong; Dian-Ping Tang; Jian-Yuan Dai
PUB. DATE
February 2005
SOURCE
Analytical & Bioanalytical Chemistry;Feb2005, Vol. 381 Issue 3, p762
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A new hydrogen peroxide biosensor was constructed, which consisted of a platinum electrode modified by a matrix of polyvinyl butyral (PVB) and nanometer-sized Ag colloid containing immobilized horseradish peroxidase (HRP), and using Co(bpy)as mediator in the hydrogen peroxide solution. The electrochemical characteristics of the biosensor were studied by cyclic voltammetry and chronoamperometry. The modified process was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The HRP immobilized on colloidal Ag was stable and retained its biological activity. The sensor displays excellent electrocatalytic response to the reduction of H2O2. Analytical parameters such as pH and temperature were also studied. Linear calibration for H2O2 was obtained in the range of 1×10-5 to 1×10-2 M under optimized conditions. The sensor was highly sensitive to H2O2, with a detection limit of 2×10-6 M, and the sensor achieved 95% of steady-state current within 10 s. The sensor exhibited high sensitivity, selectivity and stability.
ACCESSION #
16184151

 

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