Effect of central corticotropin releasing factor on hepatic circulation in rats: the role of the CRF2 receptor in the brain

Yonecla, M.; Nakamura, K.; Nakade, Y.; Tamano, M.; Kono, T.; Watonobe, H.; Shimada, T.; Hiraishi, H.; Terano, A.
February 2005
Gut;Feb2005, Vol. 54 Issue 2, p282
Academic Journal
Backgrounds: Corticotropin releasing factor (CRF) is distributed in the central nervous system and acts as a neurotransmitter to regulate gastric functions through vagal-muscarinic pathways. We have recently demonstrated that central CRF aggravates experimental acute liver injury in rats. In the present study, the central effect of CRF on hepatic circulation was investigated. Methods: Hepatic surface perfusion was determined by laser Doppler flowmetry in urethane anaesthetised rats. Portal pressure and portal blood flow was simultaneously monitored. CRF (0.1-4 nmol), urocortin II (a selective CRF2 receptor agonist 2.5-100 pmol), or saline vehicle was inlected intracisternally, and changes in hepatic circulation were observed for 120 minutes. We examined the effects of various pretreatments with K41 498, a selective CRF2 receptor antagonist, atropine, 6-hydroxydopamine, hepatic plexus denervation, or hepatic branch vagotomy, respectively. Results: Intracisternal injection of CRF (0.2-4 nmol) caused a dose dependent decrease in hepatic surface perfusion with a maximum response occurring 60 minutes post injection. Portal pressure was dose dependently elevated and portal blood flow was decreased by intracisternal CRF concurrently with the decrease in hepatic surface perfusion. These changes in hepatic circulation by intracisternal CRF were abolished by 6-hydroxydopamine and hepatic plexus denervation, but not by atropine or hepatic vagotomy. Urocortin II injected intracisternally decreased hepatic surface perfusion and elevated portal pressure at doses within the picomolar range. Intracisternal preadministration of K41 498 inhibited the effect of central CRF on the hepatic circulation. Conclusion: These data suggest that CRF acts in the brain to decrease hepatic surface perfusion and elevate portal pressure through central CRF2 receptor and sympathetic-noradrenergic pathways.


Related Articles

  • Pediatric applications of abdominal vascular Doppler: Part II. Coley, Brian // Pediatric Radiology;Oct2004, Vol. 34 Issue 10, p772 

    Ultrasound is a remarkably powerful and versatile modality for pediatric imaging, without requiring exposure to radiation or sedatives. By providing information on blood flow, Doppler sonography can reveal details about normal physiology and disease processes not discernable from gray-scale...

  • Cervical spinal cord stimulation increases cerebral cortical blood flow in an experimental cerebral vasospasm model. Karadag, Ö.; Eroglu, E.; Gürelik, M.; Göksel, H. M.; Kiliç, E.; Gültürk, S. // Acta Neurochirurgica;Jan2005, Vol. 147 Issue 1, p79 

    Background.Cerebral microcirculatory changes during cerebral vasospasm after aneurysmal subarachnoid haemorrhage (SAH) are still controversial and uncertain. The aim of our study is to demonstrate that spinal cord stimulation (SCS) augments cerebral cortical microcirculatory blood flow in an...

  • Brain care.  // Good Health (Australia Edition);May2011, p97 

    The article focuses on human brain care and improving brain function. As mentioned, brain needs a range of nutrients, fluid and energy to work properly and adhering to general healthy-eating guidelines will ensure an adequate intake of the nutrients needed for good brain health. As mentioned,...

  • Size and flow matter in the posterior circulation. Caplan, Louis R. // Journal of Neurology, Neurosurgery & Psychiatry;Oct2009, Vol. 80 Issue 10, p1 

    The author reflects on the study which discusses the blood flow analysis of cervivocranial arteries in central nervous system. The author emphasizes some important factors to be considered in the complications of blood flow such as arterial plaques, eddies and vortices, and sheer wall stress. He...

  • Reduced Arterial Circulation to the Legs in Spinal Cord Injury as a Cause of Skin Breakdown Lesions. Deitrick, George; Charalel, Joseph; Bauman, William; Tuckman, John // Angiology;Apr/May2007, Vol. 58 Issue 2, p175 

    Skin breakdown lesions (SBLs) of the legs are common in spinal cord injury (SCI). It is assumed that the cause is deficient sensitivity and immobility of the limbs, which result in areas subjected to prolonged pressures. However, poor circulation may also be a significant factor. Indeed, strong...

  • Natural History, Evaluation, and Management of Intracranial Vascular Malformations. Brown Jr., Robert D.; Flemming, Kelly D.; Meyer, Fredric B.; Cloft, Harry J.; Pollock, Bruce E.; Link, Michael L. // Mayo Clinic Proceedings;Feb2005, Vol. 80 Issue 2, p269 

    Intracranial vascular malformations are seen increasingly in clinical practice, primarily because of advances in cross-sectional brain and spinal cord imaging. Commonly encountered lesion types include arteriovenous malformations, cavernous malformations, venous malformations, dural...

  • Skin Reactions and Itch Sensation Induced by Epicutaneous Histamine Application in Atopic Dermatitis and Controls. Heyer, Gisela; Hornstein, Otto P.; Handwerker, Hermann O. // Journal of Investigative Dermatology;Oct89, Vol. 93 Issue 4, p492 

    Itch sensations and skin reactions induced by histamine iontophoresis at six different current intensities were studied in 27 atopic dermatitis (AD) patients arid 20 healthy controls. Subjective itch ratings were assessed on a visual analogue scale (VAS) for 8-mm periods after 10-sec histamine...

  • Age-Related Changes in Microvascular Blood Flow and Transcutaneous Oxygen Tension Under Basal and Stimulated Conditions. Ogrin, Rajna; Darzins, Peteris; Khalil, Zeinab // Journals of Gerontology Series A: Biological Sciences & Medical ;Feb2005, Vol. 60A Issue 2, p200 

    Background. Adequate cutaneous microvascular blood flow and tissue oxygen tension are important prerequisites for successful tissue repair. The efficacy of tissue repair decreases with age and is linked to the age-related functional decline of unmyelinated sensory neurons that are important for...

  • Relationship Between Flow-Metabolism Uncoupling and Evolving Axonal Injury After Experimental Traumatic Brain Injury. Szu-Fu Chen; Richards, Hugh K.; Smielewski, Piotr; Johnstr�m, Peter; Salvador, Raymond; Pickard, John D.; Harris, Neil G. // Journal of Cerebral Blood Flow & Metabolism;Sep2004, Vol. 24 Issue 9, p1025 

    SummaryBlood flow-metabolism uncoupling is a well-documented phenomenon after traumatic brain injury, but little is known about the direct consequences for white matter. The aim of this study was to quantitatively assess the topographic interrelationship between local cerebral blood flow (LCBF)...


Read the Article


Sign out of this library

Other Topics