TITLE

CD4+ T cell mediated intestinal immunity: chronic inflammation versus immune regulation

AUTHOR(S)
Westendorf, A. M.; Templin, M.; Geffers, R.; Deppenmeier, S.; Gruber, A. D.; Probst-Kepper, M.; Hansen, W.; Liblau, R. S.; Gunzer, F.; Bruder, D.; Buer, J.
PUB. DATE
January 2005
SOURCE
Gut;Jan2005, Vol. 54 Issue 1, p60
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Several studies have suggested that chronic inflammatory bowel disease may be a consequence of antigen specific recognition by appropriate T cells which expand and induce immunopathology. Aims: We wished to investigate whether autoreactive CD4+ T cells can initiate the disease on recognition of enterocyte specific antigens directly and if induction of mucosal tolerance occurs. Methods: Transgenic mice (VILLIN-HA) were generated that showed specific expression of haemagglutinin from influenza virus A exclusively in enterocytes of the intestinal epithelium. To investigate the impact of enterocyte specific haemagglutinin expression in an autoimmune environment, we mated VILLIN-HA mice with T cell receptor (TCR)-HA mice expressing an α/β-TCR, which recognises an MHC class II restricted epitope of haemagglutinin, and analysed the HA specific T cells for induction of autoimmunity or tolerance. Results: In VILLIN-HA×TCR-HA mice, incomplete central deletion of HA specific lymphocytes occurred. Peripheral HA specific lymphocytes showed an activated phenotype and increased infiltration into the intestinal mucosa, but not into other organs of double transgenic mice. Enterocyte specific lamina propria lymphocytes showed a dose dependent proliferative response on antigen stimulation whereas the proliferative capacily of intraepithelial lymphocytes was reduced. Mucosal lymphocytes from VILLIN-HA×TCR-HA mice secreted lower amounts of interferon γ and interleukin (IL)-2 but higher levels of tumour necrosis factor α, monocyte chemoottractant protein 1, and IL-6. Mucosal immune reactions were accompanied by broad changes in the gene expression profile with expression of proinflammatory genes, but strikingly also a remarkable set of genes discussed in the context of peripheral induction of regulatory T cells, including IL-10, Nrpp-1, and Foxp3. Conclusions: Enterocyte specific antigen expression is sufficient to trigger a specific CD4+ T cell response leading to mucosal infiltration. In our model, progression to overt clinical disease was counteracted most likely by induction of regulatory T cells.
ACCESSION #
16163226

 

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