TITLE

ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS

AUTHOR(S)
Percival, Lorraine H.; Dunckley, G.G.; Purves, H.D.
PUB. DATE
April 1970
SOURCE
Australian Journal of Experimental Biology & Medical Science;Apr1970, Vol. 48 Issue 2, p171
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Describes a technique for isoelectric focusing of proteins in polyacrylamide gels. Use of mercuric chloride in trichloroacetic acid to fix the protein in the gel; Components demonstrated in ox and pig insulin; Suggestion that proteins which are soluble at their isoelectric points can be separated in a column stabilized against thermal convection by a sucrose gradient.
ACCESSION #
16118305

 

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