Xenopus Polycomblike 2 (XPcl2) controls anterior to posterior patterning of the neural tissue

Kitaguchi, Tetsuya; Nakata, Katsunori; Nagai, Takeharu; Aruga, Jun; Mikoshiba, Katsuhiko
June 2001
Development Genes & Evolution;Jun2001, Vol. 211 Issue 6, p309
Academic Journal
A novel gene, Xenopus Polycomblike 2 (XPcl2), which encodes a protein similar to Drosophila Polycomblike was cloned and characterized. Polycomblike belongs to the Polycomb group proteins, which maintain stable expression patterns for the clustered homeotic genes in the Drosophila embryo by forming multimeric complexes on chromatin. XPcl2 shows greater amino acid sequence homology to human and mouse M96 (hPcl2, mPcl2) than Xenopus Pcl1 (XPcl1), mouse Tctex3 (mPcl1) and human PHF1 (hPcl1), indicating that at least two types of Polycomblike genes are conserved between amphibians and mammals. XPcl2 mRNA is present both maternally and zygotically, and the temporal expression profile is distinct from XPcl1, another member of the Polycomblike family in Xenopus. XPcl2 is highly expressed in the anterior-dorsal region of Xenopus following the neurula stage in a manner similar to XPcl1. Overexpression of XPcl2 disturbs the development of the anterior central nervous system, eye and cement gland. In the XPcl2-overexpressing embryo, a hindbrain marker, Krox20, and a spinal cord marker, HoxB9, are expressed more posteriorly, suggesting an alteration in the anterior-posterior patterning of the neural tissue. In addition, XPcl2 represses Zic3- and noggin-induced anterior neural markers, but not neural crest markers in animal cap explants. These results indicate that XPcl2 regulates anterior neural tissue development and the anterior-posterior patterning of the neural tissue.


Related Articles

  • The MEK-ERK pathway negatively regulates bim expression through the 3' UTR in sympathetic neurons.  // BMC Neuroscience;2011, Vol. 12 Issue 1, p69 

    The article focuses on a study on a new mechanism for the regulation of proapoptotic BH3-only protein Bim expression in sympathetic neurons by a nerve growth factor (NGF)-activated signalling pathway, the MEK-ERK. It states that the Bim protein can interact with antiapoptotic Bcl-2 proteins as...

  • A Rare Myelin Protein Zero (MPZ) Variant Alters Enhancer Activity In Vitro and In Vivo. Antonellis, Anthony; Dennis, Megan Y.; Burzynski, Grzegorz; Huynh, Jimmy; Maduro, Valerie; Hodonsky, Chani J.; Khajavi, Mehrdad; Szigeti, Kinga; Mukkamala, Sandeep; Bessling, Seneca L.; Pavan, William J.; McCallion, Andrew S.; Lupski, James R.; Green, Eric D. // PLoS ONE;2010, Vol. 5 Issue 12, p1 

    Background: Myelin protein zero (MPZ) is a critical structural component of myelin in the peripheral nervous system. The MPZ gene is regulated, in part, by the transcription factors SOX10 and EGR2. Mutations in MPZ, SOX10, and EGR2 have been implicated in demyelinating peripheral neuropathies,...

  • Generation and maintenance of Dmbx1 gene-targeted mutant alleles. Ohtoshi, Akihira; Bradley, Allan; Behringer, Richard; Nishijima, Ichiko // Mammalian Genome;Jul2006, Vol. 17 Issue 7, p744 

    Dmbx1 encodes a paired-like homeodomain protein that is expressed in neural tissues at mouse embryonic and postnatal stages. We previously generated two Dmbx1 mutant alleles, Dmbx1 − and Dmbx1 z , by homologous recombination in mouse embryonic stem (ES) cells. In this article we report...

  • A Database of microRNA Expression Patterns in Xenopus laevis. Ahmed, Ayisha; Ward, Nicole J.; Moxon, Simon; Lopez-Gomollon, Sara; Viaut, Camille; Tomlinson, Matthew L.; Patrushev, Ilya; Gilchrist, Michael J.; Dalmay, Tamas; Dotlic, Dario; Münsterberg, Andrea E.; Wheeler, Grant N. // PLoS ONE;10/27/2015, Vol. 10 Issue 10, p1 

    MicroRNAs (miRNAs) are short, non-coding RNAs around 22 nucleotides long. They inhibit gene expression either by translational repression or by causing the degradation of the mRNAs they bind to. Many are highly conserved amongst diverse organisms and have restricted spatio-temporal expression...

  • Enteric Neural Crest Differentiation in Ganglioneuromas Implicates Hedgehog Signaling in Peripheral Neuroblastic Tumor Pathogenesis. Gershon, Timothy R.; Shirazi, Arash; Qin, Li-Xuan; Gerald, William L.; Kenney, Anna M.; Nai-Kong Cheung // PLoS ONE;2009, Vol. 4 Issue 10, p1 

    Peripheral neuroblastic tumors (PNTs) share a common origin in the sympathetic nervous system, but manifest variable differentiation and growth potential. Malignant neuroblastoma (NB) and benign ganglioneuroma (GN) stand at opposite ends of the clinical spectrum. We hypothesize that a common PNT...

  • Developmental expression of XEEL, a novel molecule of the Xenopus oocyte cortical granule lectin family. Elinson, R. P.; Nagata, Saburo; Nakanishi, Misato; Nanba, Reiko; Fujita, Naoko // Development Genes & Evolution;Jul2003, Vol. 213 Issue 7, p368 

    We have isolated cDNA clones from a Xenopus laevis embryo library that encode a predicted translation product of 342 amino acids containing a signal sequence for secretion. The predicted protein has 62–70% amino acid identity with the Xenopus oocyte cortical granule lectin (XCGL), the...

  • Defining Synphenotype Groups in Xenopus tropicalis by Use of Antisense Morpholino Oligonucleotides. Rana, Amer Ahmed; Collart, Clara; Gilchrist, Michael J.; Smith, J. C. // PLoS Genetics;Nov2006, Vol. 3 Issue 5, p1751 

    To identify novel genes involved in early development, and as proof-of-principle of a large-scale reverse genetics approach in a vertebrate embryo, we have carried out an antisense morpholino oligonucleotide (MO) screen in Xenopus tropicalis, in the course of which we have targeted 202 genes...

  • Monoallelic expression of protocadherin genes. Chess, Andrew // Nature Genetics;Feb2005, Vol. 37 Issue 2, p120 

    Random monoallelic expression is known to affect a variety of autosomal genes involved in specifying cell identity. Now, the neuronally expressed protocadherins can be added to this list.

  • Molecular Characterization of the Mouse mtprd Gene, a Homologue of Human TPRD: Unique Gene Expression Suggesting Its Critical Role in the Pathophysiology of Down Syndrome1. Tsukahara, Fujiko; Urakawa, Ikuko; Hattori, Masahira; Hirai, Momoki; Ohba, Ken-ichi; Yoshioka, Toshimasa; Sakaki, Yoshiyuki; Muraki, Takamura // Journal of Biochemistry;1998, Vol. 123 Issue 6, p1055 

    We and others recently isolated a human TPRD gene, possessing a motif of the tetratricopeptide repeat (TPR), from the Down syndrome-critical region (DCR) of chromosome 21q22.2. In this study, we isolated a mouse homologue of TPRD cDNA, mtprd, and examined its expression profile in mouse embryos....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics