TITLE

Comparison of Genetic Heterogeneity of Hepatitis C Viral RNA in Liver Tissue and Serum

AUTHOR(S)
Xiaofeng Fan; Solomon, Harvey; Poulos, John E.; Neuschwander-Tetri, Brent A.; Di Bisceglie, Adrian M.
PUB. DATE
May 1999
SOURCE
American Journal of Gastroenterology;May1999, Vol. 94 Issue 5, p1347
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
OBJECTIVE: Hepatitis C virus (HCV) is known to be heterogeneous and to circulate as a group of closely related quasispecies in individual patients, although hepatic viral genetic characteristics have not been well documented. METHODS: Matched serum and liver samples were tested by reverse transcription polymerase chain reaction amplification and single stranded conformation polymorphism analysis of the hypervariable portion of the E2/NS I region of the HCV genome. The number of quasispecies was compared with the amount of HCV RNA, HCV genotyping, ,and infection with the hepatitis G virus. RESULTS: Sixteen of 40 patients had HCV RNA detectable in serum and liver. The HCV genotype was identical in serum and liver of all but one case. HCV RNA levels were approximately 10-fold higher in liver than serum. The number of HCV quasispecies in serum ranged between two and six (median 3.0) and in the liver between 2 and 19 (median 3.5, mean liver/serum ratio 1 to 6.3, median 1.8). The number of quasispecies in liver was equal to or greater than that in serum in all cases. HGV infection was found in 14 cases and did not influence serum or hepatic levels of HCV RNA. CONCLUSIONS: The number of hepatic HCV quasispecies usually exceeds that in serum, independent of the amount of HCV RNA and HCV genotype. This finding is compatible with clearance of some quasispecies from serum, but not liver, by putative neutralizing antibodies.
ACCESSION #
16070888

 

Related Articles

  • Stable hepatitis C virus RNA detection by RT-PCR during four days storage. De Moreau De Gerbehaye, Anne-Isabelle; Bodéus, Monique; Robert, Annie; Horsmans, Yves; Goubau, Patrick // BMC Infectious Diseases;2002, Vol. 2 Issue 1, p22 

    Background: Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV) RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated the stability of HCV RNA in serum and clotted blood...

  • Clinical Significance of Serum Hepatitis C Virus Titers in Patients with Chronic Type C Hepatitis. Jia-Horng Kao; Ming-Yang Lai; Pei-Jer Chen; Lih-Hwa Hwang; Chen, Wendy; Ding-Shinn Chen // American Journal of Gastroenterology;Mar1996, Vol. 91 Issue 3, p506 

    Objectives: To investigate the relationship between serum hepatitis C virus (HCV) titers and clinicopathological characteristics of chronic type C hepatitis. Methods: Serum HCV cDNA concentrations were determined by a competitive polymerase chain reaction assay in 60 Taiwanese patients with...

  • Hepatitis C Virus Not Found in Fulminant Non-A, Non-B Hepatitis. Wright, Teresa L.; Hsu, Henry; Donegan, Elizabeth; Feinstone, Stephen; Greenberg, Harry; Read, Alexandra; Ascher, Nancy L.; Roberts, John P.; Lake, John R. // Annals of Internal Medicine;7/15/91, Vol. 115 Issue 2, p111 

    Focuses on the use of a sensitive and specific polymerase chain reaction to detect hepatitis C virus (HCV) ribonucleic acid (RNA) in patients with fulminant hepatic failure of unknown cause. Factor that influenced the need to use the chain reaction; Method for analyzing polymerase chain...

  • HCV genotype determination in monoinfected and HIV co-infected patients in Cuba. Rodríguez Lay, Licel de los Angeles; Villalba, Maria Caridad Montalvo; Corredor, Marité Bello; Frómeta, Susel Sariego; Hernández, Jeny Marante; Carrera, Santiago Dueñas; Wong, Meilin Sánchez; Samada, Marcia; Núñez, Milay Bello; Alonso, Lidunka Valdes; da Silva Filho, Hermes Pedreira; Hübschen, Judith M.; Reis, Mitermayer G. // Transactions of the Royal Society of Tropical Medicine & Hygiene;Dec2012, Vol. 106 Issue 12, p711 

    Abstract: With the aim to characterize the HCV genotype distribution in Cuba, sera were collected from two subgroups: HCV-monoinfected and HCV/HIV co-infected patients. A combination of reverse transcription-PCR using genotype-specific primers, restriction fragment length polymorphism and...

  • Transcription-Mediated Amplification Is More Sensitive Than Conventional PCR-Based Assays for Detecting Residual Serum HCV RNA at End of Treatment. Comanor, Lorraine; Anderson, Frank; Ghany, Marc; Penillo, Robert; Heathcote, E. Jenny; Sherlock, Chris; Zitron, Ian; Hendricks, David; Gordon, Stuart C. // American Journal of Gastroenterology;Oct2001, Vol. 96 Issue 10, p2968 

    OBJECTIVE: In patients chronically infected with hepatitis C virus (HCV) undergoing antiviral therapy, sustained virologic response is suggested by viral clearance by end of treatment (EOT). Viral clearance is defined by nondetection of serum HCV RNA, usually by qualitative PCR-based assays with...

  • Efficiency of Measurements of Hepatitis C Virus RNA. Pogosyan, G.; Konovalova, A.; Protas, V.; Dodonova, A. // Bulletin of Experimental Biology & Medicine;Dec2015, Vol. 160 Issue 2, p264 

    Hepatitis C virus loading was evaluated using modifications of PCR product detection: terminal point fluorescent detection, real time assay, and agarose gel electrophoresis. The advantages and flaws of the real time PCR and electrophoretic detection of amplification products for evaluation of...

  • High Serum Hepatitis C Virus (HCV) RNA Load Predicts the Presence of HCV RNA in Saliva from Individuals with Chronic and Acute HCV Infection. Wang, Chia C.; Morishima, Chihiro; Minjun Chung; Engelberg, Rebecca; Krantz, Elizabeth; Krows, Meighan; Sullivan, Daniel G.; Gretch, David R.; Corey, Lawrence // Journal of Infectious Diseases;3/1/2006, Vol. 193 Issue 5, p672 

    The detection of hepatitis C virus (HCV) in saliva was studied. Twenty-three subjects with chronic HCV infection and 1 subject with acute HCV infection were enrolled in a 21-day study. Roche COBAS Amplicor and Bayer VERSANT HCV RNA qualitative assays were used. For the 23 subjects with chronic...

  • Detection of Hepatitis C Virus RNA in Normal Cervical Smears. Chia Wang; Polyak, Stephen J.; Corey, Lawrence // Clinical Infectious Diseases;7/15/2003, Vol. 37 Issue 2, p314 

    Studies the detection of hepatitis C virus (HCV) RNA in normal cervical smears. Use of real time polymerase chain reaction and fluorescence to detect hepatitis C virus; Immunomicroscopy of HCV replicons.

  • Evaluacija genotipa hepatitis C virusa i njegov utjecaj na terapijski odgovor pegiliranim interferonom α 2a (40)kD u kombinaciji sa ribavirinom. Vukobrat-Bijedić, Zora; Husić-Selimović, Amina; Radović, Svjetlana; Gornjaković, Srđan; Gogov, Bisera; Zubčević, Nadja; Bilalović, Nurija; Koluder, Nada // Medicinski Arhiv;2007, Vol. 61 Issue 4, p221 

    The aim of our study was to determine the genotypes of viral hepatitis C. We examined 54 patients with chronic hepatitis C who were treated at Gastroenterohepatology Department University of Sarajevo. We also monitored effects of therapeutical results in same group of patients. Polymerasa chain...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics