TITLE

An assay for determination of rat adrenal catechol-O-methyltransferase activity: comparison of spontaneously hypertensive rats and Wistar-Kyoto rats

AUTHOR(S)
Tsunoda, Makoto; Imai, Kazuhiro
PUB. DATE
December 2004
SOURCE
Analytical & Bioanalytical Chemistry;Dec2004, Vol. 380 Issue 7/8, p887
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A method has been developed for measurement of catechol-O-methyltransferase (COMT) activity in the rat adrenal gland. Epinephrine, synthesized in the adrenal gland, was used as substrate, and its enzymatic product, metanephrine, was quantified by high-performance liquid chromatography (HPLC) with fluorescence detection. The method has sufficient precision and accuracy. Soluble (S) and membrane-bound (MB) COMT activity in Wistar-Kyoto (WKY) rats was 20.7±3.5 and 18.6±3.4 pmol min-1 mg-1 protein (n=5), respectively. To clarify the role of adrenal COMT in blood-pressure regulation, S and MB COMT activity in spontaneously hypertensive rats were determined. Respective activity was 18.6±3.4 and 17.0±1.1 pmol min-1 mg-1 (n=5), which is similar to that in WKY rats. This finding suggests that COMT in the adrenal gland might not be related to blood pressure regulation.
ACCESSION #
16004393

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics