The Mitochondrial Sequences ofHeptathela hangzhouensisandOrnithoctonus huwenaReveal Unique Gene Arrangements and Atypical tRNAs

Qiu, Yang; Song, Daxiang; Zhou, Kaiya; Sun, Hongying
January 2005
Journal of Molecular Evolution;Jan2005, Vol. 60 Issue 1, p57
Academic Journal
We have sequenced the complete mitochondrial genomes of the spidersHeptathela hangzhouensisandOrnithoctonus huwena. Both genomes encode 13 protein-coding genes, 22 tRNA genes, and 2 ribosomal RNA genes.H. hangzhouensis, a species of the suborder Mesothelae and a representative of the most basal clade of Araneae, possesses a gene order identical to that ofLimulus polyphemusof Xiphosura. On the other hand,O. huwena, a representative of suborder Opisthothelae, infraorder Mygalomorphae, was found to have seven tRNA genes positioned differently from those ofLimulus. TherrnL-trnL1-nad1arrangement shared by the araneomorph families Salticidae, Nesticidae, and Linyphiidae and the mygalomorph family Theraphosidae is a putative synapomorphy joining the mygalomorph with the araneomorph. Between the two species examined, base compositions also differ significantly. The lengths of most protein-coding genes inH. hangzhouensisandO. huwenamtDNA are either identical to or slightly shorter than theirLimuluscounterparts. Usage of initiation and termination codons in these protein-coding genes seems to follow patterns conserved among most arthropod and some other metazoan mitochondrial genomes. The sequences of the 3' ends ofrrnSandrrnLin the two species are similar to those reported forLimulus, and the entire genes are shortened by about 100-250 nucleotides with respect toLimulus. The lengths of most tRNA genes from the two species are distinctly shorter than those ofLimulusand the sequences reveal unusual inferred tRNA secondary structures. Our finding provides new molecular evidence supporting that the suborder Mesothelae is basal to opisthothelids.


Related Articles

  • Short read Illumina data for the de novo assembly of a non-model snail species transcriptome (Radix balthica, Basommatophora, Pulmonata), and a comparison of assembler performance. Feldmeyer, Barbara; Wheat, Christopher W.; Krezdorn, Nicolas; Rotter, Bj√∂rn; Pfenninger, Markus // BMC Genomics;2011, Vol. 12 Issue 1, p317 

    Background: Until recently, read lengths on the Solexa/Illumina system were too short to reliably assemble transcriptomes without a reference sequence, especially for non-model organisms. However, with read lengths up to 100 nucleotides available in the current version, an assembly without...

  • The Evolution of tRNA-Leu Genes in Animal Mitochondrial Genomes. Higgs, Paul G.; Jameson, Daniel; Jow, Howsun; Rattray, Magnus // Journal of Molecular Evolution;Oct2003, Vol. 57 Issue 4, p435 

    Animal mitochondrial genomes usually have two transfer RNAs for leucine: one, with anticodon UAG, translates the four-codon family CUN, while the other, with anticodon UAA, translates the two-codon family UUR. These two genes must differ at the third anticodon position, but in some species the...

  • Transcription and its regulation in mammalian and human mitochondria. Sologub, M. Yu.; Kochetkov, S. N.; Temiakov, D. E. // Molecular Biology;Apr2009, Vol. 43 Issue 2, p198 

    In eukaryotic cells, mitochondria are the primary source of ATP, which is generated by oxidative phosphorylation. Mammalian and human mitochondria contain their own genome, which is maternally inherited. Defects in mitochondrial gene expression result in a number of human diseases and contribute...

  • Genome-Wide Distribution of RNA-DNA Hybrids Identifies RNase H Targets in tRNA Genes, Retrotransposons and Mitochondria. El Hage, Aziz; Webb, Shaun; Kerr, Alastair; Tollervey, David // PLoS Genetics;Oct2014, Vol. 10 Issue 10, p1 

    During transcription, the nascent RNA can invade the DNA template, forming extended RNA-DNA duplexes (R-loops). Here we employ ChIP-seq in strains expressing or lacking RNase H to map targets of RNase H activity throughout the budding yeast genome. In wild-type strains, R-loops were readily...

  • Mutations in the Drosophila Mitochondrial tRNA Amidotransferase, bene/gatA, Cause Growth Defects in Mitotic and Endoreplicating Tissues. Morris, Jason Z.; Bergman, Leah; Kruyer, Anna; Gertsberg, Mikhail; Guigova, Adriana; Arias, Ronald; Pogorzelska, Monika // Genetics;Feb2008, Vol. 178 Issue 2, p979 

    Rapid larval growth is essential in the development of most metazoans. In this article, we show that bene, a gene previously identified on the basis of its oogenesis defects, is also required for larval growth and viability. We show that all bene alleles disrupt gatA, which encodes the...

  • Read More.  // Hairy Tarantulas;2009, p24 

    The article lists books related to tarantulas, which are "Tarantula," by Edana Eckart and "Goliath Bird-Eating Tarantula," by Meish Goldish.

  • TERROR-IFIC TARANTULAS! Lambeth, Ellen // Ranger Rick;Feb2001, Vol. 35 Issue 2, p19 

    Presents information about tarantulas, a kind of spiders.

  • SPIDER MAN. Montgomery, Sy // Ranger Rick;Oct2004, Vol. 38 Issue 10, p4 

    Presents information on tarantulas.

  • TARANTULA TREAT. Bryner, Jeanna // Science World;10/23/2006, Vol. 63 Issue 4, p21 

    The article describes some of the qualities of tarantulas that make them a tasty snack.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics