TITLE

Direct visualization of nanopatterns by single-molecule imaging

AUTHOR(S)
Erwen Mei; Sharonov, Alexey; Ferris, James H.; Hochstrasser, Robin M.
PUB. DATE
January 2005
SOURCE
Applied Physics Letters;1/24/2005, Vol. 86 Issue 4, p043102
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The structures of patterned surface regions were clearly visualized by superimposing a series of single-molecule images from a total internal reflection fluorescence microscope, clearly demonstrating that a single-molecule imaging method can be used to directly visualize nanostructures. The pattern was fabricated on a microscope cover glass that formed a sandwich with a regular cover glass. The incorporated solution of fluorescent probe dyes was examined by single-molecule techniques. The effect of the surface pattern on the diffusion of the probe has also been examined.
ACCESSION #
15925559

 

Related Articles

  • Simultaneous four-color imaging of single molecule fluorophores using dichroic mirrors and four charge-coupled devices. Haga, Takanobu; Sonehara, Tsuyoshi; Sakai, Tomoyuki; Anazawa, Takashi; Fujita, Takeshi; Takahashi, Satoshi // Review of Scientific Instruments;Feb2011, Vol. 82 Issue 2, p023701 

    We developed a total-internal-reflection (TIR) fluorescence microscopy using three dichroic mirrors and four charge-coupled devices (CCDs) to detect simultaneously four colors of single-molecule (SM) fluorophores. Four spectrally distinct species of fluorophores (Alexa 488, Cy3, Cy5, or Cy5.5)...

  • Single-molecule binding experiments on long time scales. Elenko, Mark P.; Szostak, Jack W.; van Oijen, Antoine M. // Review of Scientific Instruments;Aug2010, Vol. 81 Issue 8, p083705 

    We describe an approach for performing single-molecule binding experiments on time scales from hours to days, allowing for the observation of slower kinetics than have been previously investigated by single-molecule techniques. Total internal reflection fluorescence microscopy is used to image...

  • Study of Fluidity of Lipid Membranes Using Single Molecule Microscopy. Hibino, M.; Kobayashi, M. // AIP Conference Proceedings;2004, Vol. 708 Issue 1, p326 

    The motion of single dye molecules in supported phospholipid membranes can be visualized by using total internal reflection fluorescence (TIRF) microscopy. For demonstration of the potentials of observing individual molecules we imaged a fluorescence labeled molecule in membranes made from...

  • Note: An easy way to enable total internal reflection-fluorescence correlation spectroscopy (TIR-FCS) by combining commercial devices for FCS and TIR microscopy. Yordanov, Stoyan; Best, Andreas; Weisshart, Klaus; Koynov, Kaloian // Review of Scientific Instruments;Mar2011, Vol. 82 Issue 3, p036105 

    Total internal reflection-fluorescence correlation spectroscopy (TIR-FCS) is a powerful method for studying dynamic processes at liquid-solid interfaces that may have numerous applications in biology, physics, and material science. Despite of its power and versatility, however, the use of...

  • Analysis of Synaptic-Like Microvesicle Exocytosis of B-Cells Using a Live Imaging Technique. Bergeron, Aurélie; Pucci, Luca; Bezzi, Paola; Regazzi, Romano // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    Pancreatic β-cells play central roles in blood glucose homeostasis. Beside insulin, these cells release neurotransmitters and other signaling molecules stored in synaptic-like microvesicles (SLMVs). We monitored SLMV exocytosis by transfecting a synaptophysin-pHluorin construct and by...

  • SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy. Tang, Yunqing; Dai, Luru; Zhang, Xiaoming; Li, Junbai; Hendriks, Johnny; Fan, Xiaoming; Gruteser, Nadine; Meisenberg, Annika; Baumann, Arnd; Katranidis, Alexandros; Gensch, Thomas // Scientific Reports;6/26/2015, p11073 

    Single molecule localization based super-resolution fluorescence microscopy offers significantly higher spatial resolution than predicted by Abbe's resolution limit for far field optical microscopy. Such super-resolution images are reconstructed from wide-field or total internal reflection...

  • BLAZING FUTURE Paths in TIRF. Semiatin, Ilene K. // R&D Magazine;Aug2006, Vol. 48 Issue 8, p24 

    The article reports on the developments in total internal reflection fluorescence (TIRF) microscopy, a method for viewing single molecule and cellular fluorescence at locations where molecules interact with surfaces. A development has been the introduction of high-quality chromatic correction....

  • Microscopy's Expanding Role. Karet, Gail; Studt, Tim // R&D Magazine;Aug2000, Vol. 42 Issue 8, p16 

    Discusses the impact of technology on the field of optical microscopy. Major technological changes in microscopy; Information on the optical technique called total internal reflection fluorescence microscopy; Recommendations for buying a microscope.

  • The last few milliseconds in the life of a secretory granule: Docking, dynamics and fusion visualized by total internal reflection fluorescence microscopy (TIRFM). Oheim, M.; Loerke, Dinah; Stühmer, Walter; Chow, Robert H. // European Biophysics Journal;1998, Vol. 27 Issue 2, p83 

    Abstract We have monitored single vesicles (granules) in bovine adrenal chromaffin cells using an optical sectioning technique, total internal reflection fluorescence microscopy (TIRFM). With TIR, fluorescence excitation is limited to an optical slice near a glass/water interface. In cells...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics