Direct visualization of nanopatterns by single-molecule imaging

Erwen Mei; Sharonov, Alexey; Ferris, James H.; Hochstrasser, Robin M.
January 2005
Applied Physics Letters;1/24/2005, Vol. 86 Issue 4, p043102
Academic Journal
The structures of patterned surface regions were clearly visualized by superimposing a series of single-molecule images from a total internal reflection fluorescence microscope, clearly demonstrating that a single-molecule imaging method can be used to directly visualize nanostructures. The pattern was fabricated on a microscope cover glass that formed a sandwich with a regular cover glass. The incorporated solution of fluorescent probe dyes was examined by single-molecule techniques. The effect of the surface pattern on the diffusion of the probe has also been examined.


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