TITLE

PROBIOTINIŲ PIENARŪGŠČIŲ BAKTERIJŲ LIOFILIZUOJAMŲ IR SAUGOMŲ ĮVAIRIOS APSAUGINĖSE TERPĖSE GYVYBINGUMAS

AUTHOR(S)
Kantautaitė, Jonė; Sederevičius, Antanas; Oberauskas, Vaidas; Monkevičienė, Ingrida; Sutkevičienė, Rasa; Laugalis, Jonas; Želvytė, Rasa
PUB. DATE
December 2004
SOURCE
Veterinarija ir Zootechnika;2004, Vol. 28 Issue 50, p87
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
During the process of lyophilization and after it in order to soften the negative effects of various factors (chilling, dehidratation, rehidratation, long-term preservation) on microorganisms and to maintain their activity and viability protective media of different composition are used. The strains of probiotic lactic acid bacteria L. fermentum and L. plantarum were isolated from the organism of cattle and the effect of five protective media during different stages of lyophilization and long-term preservation was studied. MRS selective broth was used for the accumulation of biomass (Liofilchem, Italy) with 2% of bacterial culture. The biomass of microorganisms concentrated during the stationary stage of growth was suspended 1:3 with protective media of certain composition (Nr 1; 2; 3; 4; 5), containing skimmed milk, various carbohydrates and other ingredients. The lyophilyzer GT-2 (Leybold-Heraeus, Vokietija) was used. After the lyophilization tightly closed products were kept 12 months at the temperature of +4°C. The number of viable lactobacillus was studied by the plate method on rigid nutritive media – MRS agar (Liofilchem, Italy): under the protective media; after freezing; after lyophilization and after 12 month storage at the temperature of +4°C. The changes of lactobacillus viability were evaluated in each protective media at the different stages of lyophilization and the results were compared to other protective media studied. It was defined that the strain L. fermentum No.4 underwent all stages of lyophilization rather satisfactory. The percent of viability survival in the medium No.4 was 81.3% comparing to the initial amount after freezing; 72% - after lyophilization; 66.5% - after 12 month storage; respectively in the medium No.5 - 82.8%; 74.8%; 69.7%. The ingredients of these protective media demonstrated positive effect on the viability of microorganisms in unfavorable environmental conditions. The best survival was found for L.plantarum in the mediun No.5 - respectively 87.6%; 76.5%; 75.3%.
ACCESSION #
15776600

 

Related Articles

  • KAI KURIŲ VEIKSNIŲ Ä®TAKA LIOFILIZUOTŲ LACTOBACILLUS PLANTARUM IR LACTOBACILLUS FERMENTUM GYVYBINGUMUI. Kantautaitė, Jonė; Sutkevičienė, Rasa; Oberauskas, Vaidas; Želvytė, Rasa; Monkevičienė, Ingrida; Laugalis, Jonas; Sederevičius, Antanas // Veterinarija ir Zootechnika;2004, Vol. 28 Issue 50, p92 

    The present study was designed to assess the impact of different protective media, the method of concentration and the age of the lactate-fermenting bacteria Lactobacillus plantarum and Lactobacillus fermentum on their viability during the lyophilization. The cultures of L. plantarum and L....

  • Probiotics or prebiotics? Turner, Natasha // Alive: Canada's Natural Health & Wellness Magazine;Nov2005, Issue 277, p50 

    The article focuses on the health benefits derived from probiotics. Beneficial bacteria found in probiotics can keep the digestive system functioning normally. Research has found intestinal microflora are cancer-protective, immune-enhancing, and anti-inflammatory. Enriching microflora with...

  • Conversion of rice straw to bio-based chemicals: an integrated process using Lactobacillus brevis. Jae-Han Kim; Block, David; Shoemaker, Sharon; Mills, David // Applied Microbiology & Biotechnology;May2010, Vol. 86 Issue 5, p1375 

    Commercialization of lignocellulosic biomass as a feedstock for bio-based chemical production is problematic due to the high processing costs of pretreatment and saccharifying enzymes combined with low product yields. Such low product yield can be attributed, in large part, to the incomplete...

  • Optimization of a protective medium for enhancing the viability of freeze-dried Lactobacillus delbrueckii subsp. bulgaricus based on response surface methodology. Lijin Huang; Zhaoxin Lu; Yongjun Yuan; Fengxia Lü; Xiaomei Bie // Journal of Industrial Microbiology & Biotechnology;Jan2006, Vol. 33 Issue 1, p55 

    Response surface methodology (RSM) was used to optimize a protective medium for enhancing the cell viability of Lactobacillus delbrueckii subsp. bulgaricus LB14 during freeze-drying. Using a previous Plackett–Burman design, it was found that sucrose, glycerol, sorbitol and skim milk were...

  • Lactobacillus.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1288 

    A definition of the term "Lactobacillus," which refers to a genus of gram-positive, anaerobic bacilli of the Lactobacillaceae family, is presented.

  • Evaluación de una estrategia de conservacion para Brevundimonas diminuta.ó. Burguet, Nancy; Sierra, Nelson // Revista VacciMonitor (Vacunología y Temas Afines);ene-abr2012, Vol. 21 Issue 1, p10 

    Brevundimonas diminuta is internationally recognized as the standard micro-organism for bacterial challenge tests. The Quality Control Laboratory of Liorad Laboratories has a collection of reference strains, among them Brevundimonas diminuta are kept by the freezing method at -70 °C,...

  • Anaerococcus.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p107 

    A definition of the term "anaerococcus" is presented which refers to a genus of anaerobic gram-positive cocci, categorized in the past as members of the genus Peptostreptococcus, or a butyrate-manufacturing saccharolytic bacteria which are sometimes pathogenic to human beings.

  • Beyond coffee. Friedman, Dorian // U.S. News & World Report;07/13/98, Vol. 125 Issue 2, p12 

    Notes advances in the use of freeze-drying. Freeze-dried, reconstituted mouse sperm; Freeze-dried migraine medication; Pet preservation.

  • Lyophilized Carnobacterium divergens AS7 bacteriocin preparation improves performance of broiler chickens challenged with Clostridium perfringens. Józefiak, D.; Sip, A.; Rutkowski, A.; Rawski, M.; Kaczmarek, S.; Wolun-Cholewa, M.; Engberg, R. M.; Højberg, O. // Poultry Science;Aug2012, Vol. 91 Issue 8, p1899 

    The present study aimed to investigate the effects of Carnobacterium divergens AS7 bacterio-cin (divercin AS7) on growth performance, digestibility, fermentation processes, selected microbial populations, and histomorphology in broiler chickens challenged with a mixture of 3 Clostridium...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics