TITLE

KIAULIŲ PLAUČIŲ PAŽEIDIMU, BŪDINGŲ ENZOOTINEI PNEUMONIJAI, VERTINIMAS IR SUKĖLĖJO MYCOPLASMA HYOPNEUMONIAE IDENTIFIKAVIMAS

AUTHOR(S)
Šiugždaitė, Jūrate; Žilinska, Henrikas; Garlaitė, Kristina
PUB. DATE
December 2004
SOURCE
Veterinarija ir Zootechnika;2004, Vol. 28 Issue 50, p31
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Mycoplasma hyopneumoniae is the primary agent of enzootic pneumonia, one of the most chronic diseases in pigs herds, with high morbidity. Diagnosis of enzootic pneumonia has been characterized by complex investigations. One of such investigations scored lung lesions by R.F. Goodwin and P. Whittlestone (1968). Lesions of mycoplasmal pneumonia are characteristic and differ from those induced by other agents (Haemophilus parasuis, Actinobacillus pleuropneumoniae, Pasteurella multocida). Total 396 pigs from nine farms (in each 44) 178 – 206 days were selected for the experiment. Lungs with gross lessions were selected for microbiological investigation. All mycoplasma cultivation procedures were performed according to the methods used in the mycoplasma section at the Danish Veterinary laboratory in Copenhagen (Friis, 1975). Isolated strains of mycoplasma were identified by the disc grow inhibition test (DGI), using antisera against the type „J“ strain of M. hyopneumoniae and strain Ms 42 of M. flocculare. M. hypneumoniae was isolated and identified by the disc grow inhibition test (DGJ) in 80,6%. RAPD analysis confirmed that M. hyopneumoniae strains, isolated by classical methods, belonged to the same M. hypneumoniae species. RAPD results showed that in different farms it was possible to obtain genetically heterogenous strains.
ACCESSION #
15776367

 

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