TITLE

Decreased expression of fibroblast growth factor-4 and associated dysregulation of trophoblast differentiation in mouse blastocysts exposed to high D-glucose in vitro

AUTHOR(S)
Leunda-Casi, A.; De Hertogh, R.; Pampfer, S.
PUB. DATE
October 2001
SOURCE
Diabetologia;Oct2001, Vol. 44 Issue 10, p1318
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aims/hypothesis: Paracrine interactions are thought to operate between the inner cell mass and trophectoderm cell lineages to co-ordinate their expansion and differentiation during embryo implantation. We aimed to determine whether hyperglycaemia could interfere with this regulatory process. Methods: Mouse blastocysts were pre-exposed to either control or high concentrations of d-glucose for 24 h in vitro and tested for their ability to attach and spread over a fibronectin-coated culture substrate. Quantitative immunocytochemistry was done on blastocysts to assess the protein expression of Fibroblast Growth Factor-4, a growth factor preferentially produced by the inner cell mass and thought to restrict trophectoderm differentiation into giant trophoblasts. Experiments were then done combining the pre-exposure to high d-glucose with the addition of recombinant fibroblast growth factor-4. Results: Compared with control blastocysts, high d-glucose pre-treated embryos were found to form 18 % larger trophoblast outgrowths. These blastocysts also showed a 30 % reduction in the expression of fibroblast growth factor-4 protein by inner cell mass cells as they were outgrowing. The trophoblast surface area per outgrowth and the trophoblast nuclear area were corrected when the addition of recombinant fibroblast growth factor-4 was combined with the pre-exposure to high d-glucose. Conclusion/interpretation: The data suggests that trophectoderm differentiation is impaired by high d-glucose and that this effect is secondary to a deficiency in fibroblast growth factor-4 protein in the inner cell mass. These observations add a novel perspective to the study of the peri-implantation embryopathy associated with maternal diabetes. [Diabetologia (2001) 44: 1318–1325]
ACCESSION #
15732616

 

Related Articles

  • Development of in Vitro Model Reveals Formation of Blastocyst-like Spheroids.  // Fertility Weekly;11/5/2012, p4 

    The article offers information on a study conducted by Jennie C. Holmberg and colleagues, on the development of an in vitro model of human uterine or trophoblast interaction and the environment of uterine implantation. It states that the model could be helpful for understanding the processes of...

  • The Role of GLUT3 in Embryonic Development.  // Fertility Weekly;5/4/2009, p8 

    The article discusses the study from Germany which identifies the defect that causes embryos to die after implantation. The glucose transporter gene Slc2a31/ (GLUT3) is known to be critical to embryonic development as GLUT3 expression is down-regulated a reduction of glucose uptake and a 50%...

  • Factors Relating to Successful Cryopreserved Blastocyst Transfer. Behr, Barry // Fertility Weekly;5/20/2002, p14 

    Reports on the viability of blastocyst transfer for the selection of embryos prior to embryo transfer or cryopreservation. Elimination of the guesswork that exist in selecting embryos; Percentage of implantation rate; Rate of preserved blastocyst.

  • Two-step consecutive transfer of early embryos and blastocysts. Kyono, Koichi; Fukunaga, Noritaka; Chiba, Setsuyo; Nakajo, Yukiko; Fuchinoue, Kohei; Yagi, Akiko; Araki, Yasuhisa // Reproductive Medicine & Biology;Sep2003, Vol. 2 Issue 3, p133 

    Aim: To evaluate and confirm the merit of two consecutive transfer attempts of early embryos and blastocysts. Methods: A total of 685 patients underwent routine in vitro fertilization (IVF) cycles. The study population consisted of three groups: (i) Group 1, early stage embryos were transferred...

  • INVITED SESSION, SESSION 10: FSA EXCHANGE LECTURE, Monday 4 July 2011 11:45 - 12:15.  // Human Reproduction;Jan2011 Supplement 1, Vol. 26 Issue suppl_1, pi18 

    No abstract available.

  • Effects on Embryo Survival Is Stage-Specific.  // Fertility Weekly;12/18/2006, p8 

    The article presents an overview of a study on effect of human papillo virus (HPV) on embryo implantation and development conducted by Andrew A. Henneberg and colleagues in the U.S. The study is aimed at evaluating the development of early stage embryos exposed to HPV DNA and to assess the...

  • Progesterone receptor requires a co-chaperone for signalling in uterine biology and implantation. Tranguch, Susanne; Smith, David F.; Dey, Sudhansu K. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Nov2006, Vol. 13 Issue 5, p651 

    Embryo implantation is absolutely dependent on the preparation of the uterus to the receptive stage and attainment by the blastocyst of implantation competency. Co-ordinated effects of progesterone and oestrogen are essential for these processes and determine the window of implantation. In...

  • Basic aspects of implantation. Guzeloglu-Kayisli1, Ozlem; Basar, Murat; Arici, Aydin // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Dec2007, Vol. 15 Issue 6, p728 

    Implantation, a critical step for establishing pregnancy, requires molecular and cellular events resulting in healthy uterine growth and differentiation, blastocyst adhesion, invasion and placental formation. Successful implantation requires a receptive endometrium, a normal and functional...

  • The expression pattern of the Qa-2 antigen in mouse preimplantation embryos and its correlation with the Ped gene phenotype. McElhinny, AS; McElhinny, Abigail S.; Kadow, N; Kadow, Natalie; Warner, CM; Warner, Carol M. // MHR: Basic Science of Reproductive Medicine;Oct98, Vol. 4 Issue 10 

    Examines the association of the preimplantation embryo development (PED) fast phenotype of blastocysts with increased number of blastomeres in trophectoderm (TE) and inner cell mass (ICM). Expression of Qa-2 antigen; Gene expression pattern of PED at messenger RNA and protein level; Comparison...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics