Absence of clonal chromosomal relationship between concomitant B-CLL and multiple myeloma – a report on two cases

Kaufmann, H.; Ackermann, J.; Nösslinger, T.; Krömer, E.; Zojer, N.; Schreiber, S.; Urbauer, E.; Heinz, R.; Ludwig, H.; Huber, H.; Drach, J.
August 2001
Annals of Hematology;Aug2001, Vol. 80 Issue 8, p474
Academic Journal
B-cell chronic lymphocytic leukemia (B-CLL) and multiple myeloma (MM) are chronic B-cell malignancies that represent different stages of B-cell maturation. Occasionally, both diseases are present in the same patient, and this raises the question of clonal associations between the two neoplasms. We here report on two patients with concomitant B-CLL and MM. Clonal chromosomal abnormalities in both lymphocytic cells and plasma cells were studied by interphase fluorescence in situ hybridization (FISH) using a panel of 24 chromosome- and region-specific DNA probes. In the first patient, cytogenetics revealed 47, X, t(Y;22)(p11;q10), +12, del14(q21q32). By FISH, +12 was present in lymphoid cells, but not in plasma cells. MM cells were characterized by multiple chromosomal gains (1, 11q23) and losses (5q, 10, 13q14, 15, 17p13, Y), which were all undetectable in lymphoid cells. The second patient, in whom no clonal abnormalities were obtained by conventional cytogenetic analysis, had lymphoid cells with loss of 8q24 by FISH. In contrast, evidence for a gain of 8q24 (consistent with amplification of c-myc) was obtained in 13% of plasma cells. Plasma cells were further characterized by gains of chromosomes 1, 3, 11, 18, and Y. We thus conclude that this comprehensive molecular cytogenetic analysis demonstrates the existence of two clonally distinct B-cell malignancies in both patients.


Related Articles

  • Microsatellite instability in patients with chronic B-cell lymphocytic leukaemia. Niv, E.; Bomstein, Y.; Yuklea, M.; Lishner, M. // British Journal of Cancer;4/25/2005, Vol. 92 Issue 8, p1517 

    The purpose of our study was to evaluate the microsatellite instability (MSI) at selected loci with known involvement in the oncogenesis of chronic B-cell lymphocytic leukaemia (B-CLL). DNA from B cells (tumour cells) and from T cells (normal controls) of 27 samples of 26 patients with...

  • Bcl-6 mutation status provides clinically valuable information in early-stage B-cell chronic lymphocytic leukemia. Sarsotti, E.; Marugan, I.; Benet, I.; Terol, M. J.; Sanchez-Izquierdo, D.; Tormo, M.; Rubio-Moscardo, F.; Martinez-CIiment, J. A.; Garcia-Conde, J. // Leukemia (08876924);Apr2004, Vol. 18 Issue 4, p743 

    In B-cell chronic lymphocytic leukemia (B-CLL), somatic mutation of IgVH genes defines a subgroup with favorable prognosis, whereas the absence of IgVH mutations is correlated with a worse outcome. Mutations of the BCL-6 gene are also observed in a subset of B-CLL, but the clinical significance...

  • Treatment of primary CLL cells with bezafibrate and medroxyprogesterone acetate induces apoptosis and represses the pro-proliferative signal of CD40-ligand, in part through increased 15dΔ12,14,PGJ2. Hayden, R. E.; Pratt, G.; Davies, N. J.; Khanim, F. L.; Birtwistle, J.; Delgado, J.; Pearce, C.; Sant, T.; Drayson, M. T.; Bunce, C. M. // Leukemia (08876924);Feb2009, Vol. 23 Issue 2, p292 

    B-cell chronic lymphocytic leukemia (CLL), the most common leukemia in older adults, remains largely incurable and novel treatments are urgently required. We previously reported powerful pro-apoptotic actions of bezafibrate (BEZ) and medroxyprogesterone acetate (MPA) against Burkitts lymphoma...

  • Clinical Prognostic Biomarkers in Chronic Lymphocytic Leukemia and Diffuse Large B-Cell Lymphoma. Chastain, Elizabeth C.; Duncavage, Eric J. // Archives of Pathology & Laboratory Medicine;May2015, Vol. 139 Issue 5, p602 

    Context.—Diffuse large B-cell lymphoma and chronic lymphocytic leukemia are 2 of the most common B-cell lymphomas in adults. Both diffuse large B-cell lymphoma and chronic lymphocytic leukemia share heterogeneous outcomes, and the use of prognostic biomarkers to better stratify risk in...

  • Translocation t(14;18) and gain of chromosome 18/BCL2: effects on BCL2 expression and apoptosis in B-cell non-Hodgkin's lymphomas. Galteland, E.; Sivertsen, E.A.; Svendsrud, D.H.; Smedshammer, L.; Kresse, S.H.; Meza-Zepeda, L.A.; Myklebost, O.; Suo, Z.; Mu, D.; DeAngelis, P. M.; Stokke, T. // Leukemia (08876924);Dec2005, Vol. 19 Issue 12, p2313 

    Gain of chromosome 18q and translocation t(14;18) are] frequently found in B-cell non-Hodgkin's lymphomas (B-NHL). Increased BCL2 transcription and BCL2 protein expression have been suggested to be the result of the gain. We utilized FISH, PCR and array CGH to study BCL2 and chromosome 18 copy...

  • An additional segment at 1p36 derived from der(18)t(14;18) in patients with diffuse large B-cell lymphomas transformed from follicular lymphoma. Nomura, Kenichi; Kanda-Akano, Yumiko; Shimizu, Daisuke; Okuda, Takashi; Yoshida, Naohisa; Matsumoto, Yosuke; Nishida, Kazuhiro; Taki, Tomohiko; Yokota, Shohei; Horiike, Shigeo; Taniwaki, Masafumi // Annals of Hematology;Jul2005, Vol. 84 Issue 7, p474 

    The particular translocation in follicular lymphomas (FLs) is a t(14;18)(q32;q21), recombining the immunoglobulin heavy chain (IgH) gene on chromosome 14 with the B-cell leukemia/lymphoma 2 (BCL2) gene on chromosome 18. Some low-grade FLs are aggressively transformed into diffuse large B-cell...

  • BCL6 Expression Correlates With the t(1;19) Translocation in B-Lymphoblastic Leukemia. Deucher, Anne M.; Zhongxia Qi; Jingwei Yu; George, Tracy I.; Etzell, Joan E. // American Journal of Clinical Pathology;2015, Vol. 143 Issue 4, p547 

    Objectives: Study to date suggests that BCL6 protein expression in B-cell neoplasia predominates in germinal center-derived tumors, but less is known regarding its expression in B-lymphoblastic leukemia. Therefore, we designed a comprehensive study of BCL6 expression in B-lymphoblastic leukemia....

  • Cancer Stem Cells of Differentiated B-Cell Malignancies: Models and Consequences. Gross, Emilie; Quillet-Mary, Anne; Ysebaert, Loic; Laurent, Guy; Fournie, Jean-Jacques // Cancers;2011, Vol. 3 Issue 2, p1566 

    The concept of cancer stem cells has revolutionized our current vision of cancer development and was validated in solid tumors and cancers of the primitive hematopoietic compartment. Proof of the principle is still lacking, however, in malignancies of differentiated B-cells. We review here the...

  • Telomere length identifies two different prognostic subgroups among VH-unmutated B-cell chronic lymphocytic leukemia patients. Ricca, I.; Rocci, A.; Drandi, D.; Francese, R.; Compagno, M.; Lobetti Bodoni, C.; De Marco, F.; Astolfi, M.; Monitillo, L.; Vallet, S.; Calvi, R.; Ficara, F.; Omedè, P.; Rosato, R.; Gallamini, A.; Marinone, C.; Bergui, L.; Boccadoro, M.; Tarella, C.; Ladetto, M. // Leukemia (08876924);Apr2007, Vol. 21 Issue 4, p697 

    Some evidences suggest that telomere restriction fragment length (TRF-L) is an effective indicator of histopathogenesis in B-cell tumors. As histopathogenesis is relevant for B-cell chronic lymphocytic leukemia (B-CLL) prognosis, TRF-L was assessed by Southern blot in 201 patients and compared...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics